王志福 杨意州 刘建波1，李长征 龚德贵 俞向梅

摘要：目的 觀察电针对糖尿病周围神经病变（DPN）大鼠脊髓脂氧合酶（LOX）的影响，探讨其干预DPN的作用机制。方法 30只实验大鼠随机分为正常组、模型组、电针组、脂氧素组、黄芩素组，每组6只，模型组大鼠采用单次腹腔注射链脲佐菌素造模，尾静脉采血检测血糖，筛选DPN动物模型，不干预。电针组大鼠选取“肾俞”“足三里”电针2周；脂氧素组大鼠脊髓鞘内注射脂氧素3次；黄芩素组大鼠脊髓鞘内注射黄芩素3次。电子佛莱毛检测大鼠机械痛行为学，RT-PCR检测大鼠LOX基因水平，ELISA检测大鼠坐骨神经髓鞘碱性蛋白（MBP）表达。结果 与正常组比较，模型组大鼠机械痛阈值及坐骨神经MBP含量显著降低（P<0.01），大鼠脊髓5-LOX、12-LOX、15-LOX mRNA水平显著升高（P<0.01）；与模型组比较，电针组、脂氧素组和黄芩素组大鼠机械痛阈值和坐骨神经MBP含量显著升高，脂氧素组大鼠脊髓5-LOX mRNA表达显著降低（P<0.01），电针组和黄芩素组大鼠脊髓5-LOX、12-LOX、15-LOX mRNA表达显著降低（P<0.01）。结论 电针能减轻DPN大鼠痛敏反应，修复坐骨神经损伤，其机制可能与抑制脊髓LOX活性有关。

关键词：糖尿病周围神经病变；电针；脊髓；脂氧合酶；大鼠

DOI：10.3969/j.issn.1005-5304.2018.05.013

中图分类号：R245 文献标识码：A 文章编号：1005-5304（2018）05-0056-05

Effects of Electroacupuncture Therapy on Expression of Spinal Lipoxygenase

in Rats of Diabetic Peripheral Neuropathy

WANG Zhi-fu1， YANG Yi-zhou1， LIU Jian-bo1， LI Chang-zheng1， GONG De-gui2， YU Xiang-mei1

1. Integrative Medicine College， Fujian University of Traditional Chinese Medicine， Fuzhou 350122， China；

2. University of Traditional Chinese Medicine Subsidiary Rehabilitation Hospital， Fuzhou 350003， China

Abstract： Objective To observe the effects of electroacupuncture therapy on the expression of spinal lipoxygenase （LOX） in rats of diabetic peripheral neuropathy （DPN）； To discuss its mechanism in intervening DPN. Methods Thirty experimental rats were randomly divided into five groups： normal， DPN， electroacupuncture， LXA4 and Baicalein， 6 rats in each group. The model group was injected intraperitoneally with streptozotocin （STZ）， and the animal model of DPN was screened by blood sampling of tail vein with blood glucose， without intervention. Electroacupuncture was applied to bilateral “Shenshu” and “Zusanli” acupoints in electroacupuncture group for two weeks. The LXA4 and Baicalein groups were successfully intrathecal injected 3 times with LXA4 and Baicalein in the DPN rats. The paw withdrawal threshold was measured with electronic Frye fibers. The mRNA levels of LOX in the L4～5 spinal cord of DPN rats were detected with real-time PCR analysis. The protein concentration of sciatic nerve MBP was detected with ELISA analysis. Results Compared with the normal group， the mechanical pain threshold and sciatic nerve MBP content in the model group significantly decreased （P<0.01）， and the levels of 5-LOX， 12-LOX and 15-LOX mRNA in the spinal cord of the model group significantly increased （P<0.01）. Compared with model group， the mechanical pain threshold and MBP content of sciatic nerve in electroacupuncture group， LXA4 group and Baicalein group significantly increased， and the 5-LOX mRNA level in spinal cord of LXA4 group significantly

基金项目：国家自然科学基金（81774385、81704149）；福建省自然科学基金（2016J01391）；福建省教育厅中青年教师科研项目（JA15250）；福建省高校杰出青年科研人才项目（2015年）；福建省卫计委青年科研课题项目（2015-1-79）；福建省自然科学基金青年创新项目（2015J05163）

通讯作者：俞向梅，E-mail：yxmtcm@163.com

decreased （P<0.01）. The expressions of 5-LOX， 12-LOX and 15-LOX mRNA in electroacupuncture group and Baicalein group significantly decreased （P<0.01）. Conclusion Electroacupuncture therapy may reduce the pain response and restore the impairment of sciatic nerve in DPN rats through inhibiting the gene expression of LOXs in spinal cord.

Keywords： diabetic peripheral neuropathy； electroacupuncture； spinal cord； lipoxygenase； rats

糖尿病周围神经病变（diabetic peripheral neuropathy，DPN）是糖尿病常见并发症之一，在糖尿病患者中发病率约20%。临床表现为肢体远端明显疼痛、麻木，严重者可致残；DPN实验大鼠表现为热痛觉过敏、机械痛觉超敏或者触觉迟钝等感觉异常情况。DPN与其他神经病理性疼痛发病基础不同，治疗棘手，甚至出现对各类西药的抵抗，长期应用西药治疗有一定的不良反应。针刺疗法基于中医辨证论治原则，治疗DPN安全有效，临床研究表明，电针对急慢性痛均有镇痛、促进周围神经损伤修复作用[1]。……