曹婷婷　刘久石　高石曼　孙恬　覃婕媛　景浩　齐耀东　张本刚　刘海涛　肖培根

[摘要]采用HPLCELSD建立了党参药材中具有“甜味”的3种游离态糖类成分的含量测定方法，为补充和完善党参药材的质量控制提供科学依据。方法学考察结果显示蔗糖、葡萄糖、果糖分别在2312 5～18500 0，1500 0～12000 0， 2000 0～16000 0 μg呈良好的线性关系，平均加样回收率分别为9631%，1004%，1018%。该方法操作简便，稳定可靠，结果准确，适合党参药材中蔗糖、葡萄糖、果糖3种游离态糖类成分的同时检测。应用建立的分析方法对甘肃省岷县党参药材规范化种植基地不同栽培措施种植的党参药材样品进行分析，发现不同栽培措施对党参药材中3种游离态糖类成分具有一定的影响，其中，按照蔗糖含量的高低顺序为：施用壮根灵>不施用壮根灵，不打尖、搭架>不打尖、不搭架>打尖、不搭架>打尖、搭架；按照葡萄糖、果糖含量的高低顺序为：不施用壮根灵>施用壮根灵，打尖、搭架>不打尖、不搭架>不打尖、搭架>打尖、不搭架。因不同游离态糖类成分甜度和含量均具有差异性，因此可考虑将游离态糖类成分的含量作为党参药材的评价指标。

[关键词]党参； 栽培措施； 游离态糖； 含量测定

[Abstract]To provide a scientific evidence for the quality control of Codonopsis Radix， a method was established for determining the content of three free carbohydrates of Codonopsis Radix The developed method showed good linearity The calibration curves were linear within the range of 2312 518500 0 μg for sucrose， 1500 012000 0 μg for glucose， and 2000 016000 0 μg for fructose， resgectwely The recoveries varied between 9631%1018% The method is simple， accurate and reproducible， and can be used for determining the content of sucrose， glucose and fructose of Codonopsis Radix The results showed that different cultivation measures had an effect on the content of three free carbohydrates of Codonopsis Radix According to the content of sucrose， using Zhuanggenling>not using Zhuanggenling While， not pinching， shelving>not pinching， not shelving>pinching， not shelving>pinching， shelving According to the content of glucose and fructose， not using Zhuanggenling>using Zhuanggenling While， pinching， shelving>not pinching， not shelving>not pinching， shelving>pinching， not shelving In consideration of the differences of sweetness and content of the three free carbohydrates in Codonopsis Radix， we recommend that the content of free carbohydrates could be considered as the marker to evaluate the quality of Codonopsis Radix.

[Key words]Codonopsis Radix； cultivation measures； free carbohydrates； quantitative analysis

黨参为桔梗科植物党参Codonopsis pilosula （Franch） Nannf、素花党参C pilosula Nannf var modesta （Nannf） L T Shen或川党参C tangshen Oliv的干燥根，具有健脾益肺，养血生津等功效，主要用于治疗脾肺气虚，食少倦怠，咳嗽虚喘，气血不足等症[1]，其化学成分主要有三萜、糖类、甾类、木脂素、倍半萜、黄酮等[23]。现代药理研究表明，党参可以显著提高机体免疫力，具有促进造血机能、清除自由基、增强机体免疫力、延缓衰老、抗肿瘤、调节胃收缩及抗溃疡等多种作用[45]。