申文雯　张银　仇守蓓　朱粉霞　贾晓斌　汤道权　陈斌

[摘要]采用超高效液相色谱与串联四极杆飞行时间质谱仪联用技术（UPLCQTOFMS/MS）分析大鼠灌胃蒸三七正丁醇部位后所得血液、尿液和粪便样品，获取正负离子模式下样品中化学成分的分析数据。样品数据经PeakView软件处理后，根据原型化合物的结构特征以及体内常见代谢方式的准确质量数变化，对代谢产物进行解析。最终利用代谢物的MS/MS信息鉴定了蒸三七正丁醇部位中的4个皂苷代谢原型，即人参皂苷Rh4，Rk3，Rk1，Rg5及其15个代谢产物。蒸三七正丁醇部位中4个人参皂苷的代谢途径包括葡萄糖醛酸化、脱糖、硫酸化、脱羟甲基化、支链丢失等。该文从定性角度对蒸三七主要活性皂苷类组分在体内的代谢开展了研究，进一步阐明蒸三七在体内发挥药效的物质基础。

[关键词]蒸三七正丁醇部位； 代谢产物鉴定； UPLCQTOFMS/MS； 人参皂苷Rh4，Rk3，Rk1，Rg5； 转化方式

[Abstract]UPLCQTOFMS/MS was used to identify metabolites in rat blood， urine and feces after the administration of nbutanol extract derived from steamed notoginseng The metabolic process of saponins came from steamed notoginseng was analyzed The metabolites were processed by PeakView software， and identified according to the structural characteristics of prototype compounds and the accurate qualitative and quantitative changes of common metabolic pathways Four saponins metabolites were identified based on MS/MS information of metabolites， namely ginsenoside Rh4， Rk3， Rk1， Rg5，and their 15 metabolites were verified The metabolic pathways of the four ginsenosides in nbutanol extract included glucuronidation， desugar， sulfation， dehydromethylation， and branch loss The metabolites of main active saponin components derived from steamed Panax notoginseng were analyzed from the perspective of qualitative analysis And the material basis for the efficacy of steamed notoginseng was further clarified.

[Key words]nbutanol extract derived from Panax notoginseng； identification of metabolites； UPLCQTOFMS/MS； ginsenoside Rh4， Rk3， Rk1， Rg5； transformation mode

三七為五加科植物三七Panax notoginseng （Burk.）F.H.Chen的干燥根。蒸三七作为三七中的一种炮制品，应用广泛，具有滋养血液、增强免疫功能、抑制肿瘤、增强健壮性、促进组织再生等功效，古人朴素认识为“熟补”。现代研究表明，三七炮制前后成分变化差异较大[1]。课题组前期研究发现，蒸三七中含有大量的不同于生三七的皂苷类化合物，各皂苷成分是其发挥补血功效的真正物质基础[2]，药理试验表明以人参皂苷Rg3，Rg6，F4，Rh4，Rk3，Rk1，Rg5等为代表的皂苷类化合物具有增强免疫功能、抑制肿瘤等多种生物活性[37]。对蒸三七主要活性皂苷组分在体内过程的定性研究，有利于揭示蒸三七主要活性部位在体内发挥药效的物质基础及作用机制。……