李益群　徐丽　朱华旭　唐志书　李博　潘永兰　姚薇薇　付廷明　郭立玮

[摘要]为探索蛋白质在膜表面的吸附特性以及蛋白质存在的溶液环境对小檗碱膜透过行为的影响，以蛋白质、小檗碱为研究对象，配制模拟溶液，通过低场核磁共振技术、静态吸附实验以及膜分离实验探究蛋白质与陶瓷膜、小檗碱之间可能存在的相互作用，以蛋白质静态吸附量、膜过程相对通量、蛋白质截留率、小檗碱透过率及两者的吸附率为评价指标，同时测定膜过程中膜污染阻力分布、过膜前后粒径分布变化及膜表面电镜扫描（SEM），研究蛋白质在陶瓷膜上的吸附特性及对小檗碱膜过程的影响。结果表明，陶瓷膜对蛋白质具有吸附作用，且吸附模型符合Langmuir吸附模型，在模拟溶液的膜分离过程中，蛋白质是引起膜污染的主要因素，但在1 g·L-1蛋白质的浓度下，蛋白质的存在对小檗碱的膜过程没有显著影响。

[关键词]蛋白质； 小檗碱； 陶瓷膜微滤； 模拟体系； 吸附特性

[Abstract]In order to explore the adsorption characteristics of proteins on the membrane surface and the effect of protein solution environment on the permeation behavior of berberine， berberine and proteins were used as the research object to prepare simulated solution Low field NMR， static adsorption experiment and membrane separation experiment were used to study the interaction between the proteins and ceramic membrane or between the proteins and berberine The static adsorption capacity of proteins， membrane relative flux， rejection rate of proteins， transmittance rate of berberine and the adsorption rate of proteins and berberine were used as the evaluation index Meanwhile， the membrane resistance distribution， the particle size distribution and the scanning electron microscope （SEM） were determined to investigate the adsorption characteristics of proteins on ceramic membrane and the effect on membrane separation process of berberine The results showed that the ceramic membrane could adsorb the proteins and the adsorption model was consistent with Langmuir adsorption model In simulating the membrane separation process， proteins were the main factor to cause membrane fouling However， when the concentration of proteins was 1 g·L-1， the proteins had no significant effect on membrane separation process of berberine.

[Key words]protein； berberine； ceramic membrane microfiltration； simulation system； adsorption characteristics

陶瓷膜具有耐高温、耐腐蚀、机械强度高、化学稳定性好等特点，在生物醫药、石油化工、食品饮料、水处理等领域得到广泛的应用[12]，尤其是在中药领域，凭借其对于中药水提液分离纯化的独特优势而具有广阔的应用前景[34]，但中药水提液组成复杂多样，小分子药效物质的透过率不理想，严重制约了陶瓷膜在中药领域的推广应用，又缺少系统的研究方法，尤其是缺乏对高分子与膜、小分子与膜、高分子与小分子、高分子与高分子之间相互作用的研究手段，所以对膜过程机制的研究难以深入。……