郑涛+郑域茹+危天进+林晓兰+武英+李发林

摘 要 應用JIP-test初步分析了不同溶液处理对柚苗叶片光合PSⅡ系统的影响，结果表明：处理后柚叶Fo升高，Fm、Fv值降低，Fv/Fm、Fv/Fo、Fm/Fo比值均降低；经百喜草、圆叶决明浸提液处理的柚苗，其叶片Fo增加幅度较小，Fm、Fv值降低幅度较小，表明2个处理PSⅡ光合色素吸收能量后，以热能和荧光形式散失的部分较少，有较多的能量可以用于下一步电子传递和能量转化，受体侧电子传递能够较为顺利进行，而2个浓度除草剂溶液处理后Fo、Fm、Fv变化幅度均最大，表明该处理使叶片吸收能量的一大部分（37.47%、40.61%）以各种形式散失，电子传递阻力较大；各处理对柚叶PSⅡ潜在活性（Fv/Fo）及捕获激发能效率（Fm/Fo）的影响要远大于对原初光能转化效率（Fv/Fm）的影响，各处理对原初光能的转化效率影响范围在5.98%～16.95%（不含CK），百喜草、圆叶决明浸提液处理对柚叶PSⅡ原初光能转化效率的影响最小，2个浓度除草剂处理Fv/Fo、Fm/Fo值大幅降低，对柚叶PSⅡ的功能伤害程度较大，严重影响了光合作用的进行。

关键词 JIP-test ；柚 ；果园生草

中图分类号 S666.3 文献标识码 A Doi：10.12008/j.issn.1009-2196.2017.09.001

Analysis of the Effects of Different Solution Treatments

on Pomelo Seedlings by using JIP-test

ZHENG Tao1） ZHENG Yuru1） WEI Tianjin2） LIN Xiaolan1） WU Ying1） LI Falin1）

（1 Fujian Institute of Tropical Crops， Zhangzhou， Fujian 363001；

2Wuping Soil and Fertilizer Station of Fujian Province， Longyan， Fujian 364300）

Abstract The effects of different solution treatments on the PS II system of pomelo were analyzed by using JIP-test. The results showed that the Fo of pomelo leaves increased， that the Fm and Fv reduced， and that the ratios of Fv/Fm， Fv/Fo and Fm/Fo decreased. The Fo increased in a small extent in the leaves of pomelo seedlings treated with the solution of the extracts of Paspalum natatum and Chamaecrista rotundifolia， and the Fm and Fv decreased very little. This indicated that after the PS II system of the leaves treated with these two extract solutions absorbed energy， more of this energy were used for the next step of electron transport and energy conversion； only less energy dissipated in the forms of heat energy and fluorescence； and， the electron transport could be conducted in the receptor side. The change of Fo， Fm and Fv of the leaves of the seedlings treated with two herbicides were the highest. This result showed that most of the energy absorbed by the leaves treated with two herbicides （37.47%， 40.61%） were lost in various forms and that the electron transport was suffocated. All the treatments had much higher effect on the Fv/Fo and Fm/Fo than the Fv/Fm. The efficiency of primary conversion of light energy （Fv/Fm） ranged between 5.98%～16.95% in all the treatments except the CK. The treatment with the extracts of P. natatum and C. rotundifolia had a minimum influence on transformation efficiency of primary optical energy. The Fv/Fo and Fm/Fo was reduced sharply after the leaves were treated with herbicides. This showed that the treatment of herbicides damaged the PSII system and affected badly the photosynthesis of the leaves treated.endprint

Keywords JIP-test ； pomelo ； weed in the orchard

JIP-test（JIP-测定）是以生物膜能量流动为基础而建立的一种分析方法，利用该方法可以快速获得有关PSⅡ（光系统Ⅱ）的基本信息，从而分析不同环境条件、处理水平等因素对植物光合反应功能、机构，如PSⅡ光能分配、供体侧、受体侧、反应中心等的影响[1]。植物光合机构包含光系统Ⅰ和光系统Ⅱ，即PSⅠ和PSⅡ，Kautsky等[2]最先發现，经过暗适应的光合材料在接受光照后，叶绿素荧光值先上升至最大（P点），然后逐渐下降，最终达到稳定，从接受光照的初始点（O点）至峰值（P点）整个过程有着丰富的荧光变化信息，其中经过了2个拐点，分别是J点和I点，4个点一般称为荧光诱导动力学曲线的O、J、I、P相[3]。……