王 波,周涧楠,黄忠勤,丁震乾,常 勇,苏在兴,周兴根

(江苏徐淮地区徐州农业科学研究所,江苏 徐州 221131)

一株多粘类芽孢杆菌Paenibacilluspolymyxa对甘薯黑斑病的生物防治效果及作用机理初探

王 波,周涧楠,黄忠勤,丁震乾,常 勇,苏在兴,周兴根*

(江苏徐淮地区徐州农业科学研究所,江苏 徐州 221131)

XZ-2是本课题组分离到的一株大豆内生多粘类芽孢杆菌。探索了该菌株对甘薯黑斑病的生物防治效果及其作用机理。研究结果表明：与清水对照相比，用XZ-2发酵原液的10～100倍稀释液处理贮藏期薯块，可以极显著地减小黑斑病病斑体积；在所有处理中，以XZ-2发酵原液的10倍稀释液对甘薯黑斑病的防治效果最佳,达73.62%；XZ-2发酵滤液处理对甘薯黑斑病菌的菌丝形态没有明显的影响，但会导致该病菌菌丝产生厚垣孢子，且显著地抑制其分生孢子的萌发。

多粘类芽孢杆菌;甘薯；黑斑病;生物防治效果；作用机理

Abstract：PaenibacilluspolymyxaXZ-2 is a biological control strain isolated from the healthy soybean plants. The biocontrol efficacy of XZ-2 against black rot of sweet potato and its action mechanism were explored. The results showed that: in comparison with the control (pure water), treating the stored sweet potatoes with the 10～100-fold diluent of XZ-2 fermentation broth could very significantly reduce the scab volume of black rot; among all treatments, the 10-fold diluent of XZ-2 fermentation broth had the best control efficacy against black rot of sweet potato, reaching 73.62%; the treatment with the diluent of XZ-2 fermentation broth had no obvious influence on the hyphal morphology of sweet potato black rot pathogen (Ceratocystisfimbriata), but it could cause the generation of chlamydospores, and could significantly inhibit the germination of conidia of this pathogen.

Keywords：Paenibacilluspolymyxa; Sweet potato; Black rot; Biocontrol efficacy; Action mechanism

多粘类芽孢杆菌Paenibacilluspolymyxa是一种能产生芽孢的革兰氏阳性菌,该菌对人畜无致病性。多粘类芽孢杆菌可产生多种抗菌蛋白[1-2]、多粘菌素[3]、核苷类[4]、酚类[5]以及吡嗪类化合物[6]等抗菌物质以及IAA等植物生长调节剂[5]。近年来,有关多粘类芽孢杆菌用于植物抗病和植物促生等方面的研究报道很多,如Hong等(2016)报道了一株植物根围促生菌多粘类芽孢杆菌菌株AC-1可抑制PseudomonassyringaeDC3000的生长并在拟南芥叶片中成功定殖,还可诱导PR-1、FRK1等抗病相关基因的表达[7];Kim等(2016)将根围拮抗细菌多粘类芽孢杆菌菌株APEC128用于苹果炭疽病的防治,其对该……