白伟良，宋岩，王枭维，李婷，项清华，周倩，李进兴

（中国医科大学附属盛京医院耳鼻咽喉科，沈阳 110004）

鼻息肉的全基因表达谱

白伟良，宋岩，王枭维，李婷，项清华，周倩，李进兴

（中国医科大学附属盛京医院耳鼻咽喉科，沈阳 110004）

目的研究鼻息肉的全基因表达谱情况，探寻其与正常鼻腔黏膜的基因表达差异。方法提取鼻息肉中的RNA，将RNA合成双链的cDNA，将标记的cDNA在Roche NimbleGen基因芯片上进行杂交。应用Axon GenePix 4000B scanner进行扫描，应用NimbleScan软件进行图像分析，探针及基因水平标准化后进行计算。结果与正常鼻腔黏膜相比，鼻息肉中有1 000个基因表达上调，占2.22% （1 000/45 033），有1 123个基因表达下调，占2.49%（1 123/45 033）。经过信号通路分析，发现在核糖体、蛋白酶体、柠檬酸循环、膀胱癌、非小细胞肺癌、神经胶质瘤、子宫内膜癌、内质网蛋白合成、慢性髓样白血病、谷胱甘肽代谢等信号通路中相关基因表达上调，在嗅觉传导、自然杀伤细胞介导的细胞毒性、细胞色素P450的化合物代谢、细胞色素P450的药物代谢、抗原加工和呈递、疟疾、移植物抗宿主疾病、视黄醇代谢、亚油酸代谢、戊糖和葡萄糖醛酸转换等信号通路中相关基因表达下调。结论鼻息肉的发生、发展与多个基因及信号通路的表达升高或降低有关，鼻息肉全基因表达谱的研究为探求鼻息肉的发生机制

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鼻息肉； 基因芯片； 基因表达； 信号通路

Abstract ObjectiveTo analyze the gene expression profiles of nasal polyp and gene expression differences between the nasal polyp and normal nasal mucosa.MethodsTotal RNA from nasal polyp tissues was purified and synthesized into double-stranded cDNA.The cDNA was labeled and hybridized in a NimbleGen hybridization chamber. The slides were scanned using the Axon GenePix 4000B microarray scanner. Scanned images were analyzed using NimbleScan software. The probes and gene levels were standardized and calculated.ResultsCompared with normal nasal mucosa tissues，expression of 2.22% （1 000/45 033） of genes was up-regulated in all cases of nasal polyp tissues，while 2.49% （1 123/45 033） of genes were down-regulated in all cases of nasal polyp tissues. We found genes related to the ribosome，proteasome，citrate cycle （TCA cycle），bladder cancer，non-small cell lung cancer，glioma，endometrial cancer，protein processing in the endoplasmic reticulum，chronic myeloid leukemia，and glutathione metabolism were up-regulated.Genes related to olfactory transduction，natural killer cell-mediated cytotoxicity，metabolism of xenobiotics by cytochrome P450，drug metabolism-cytochrome P450，antigen processing and presentation，malaria，graft-versus-host disease，retinol metabolism，linoleic acid metabolism，and pentose and glucuronate interconversions were down-regulated.ConclusionMultiple genes or pathways may be involved in the occurrence and development of nasal polyp. Gene expression profiling provides insight into the mechanism of nasal polyp development.

Keywordsnasal polyp； gene chip； gene expression； signaling pathway

鼻息肉本质上是一种炎症疾病，是一个多步骤、多因素、序贯的复杂过程，目前已发现涉及到多种基因及产物的改变，多种信号通路相互调节和拮抗［1］。……