王陈萍　许波　蔡向明

[摘要] 目的 分别评价五味子甲素、五味子乙素、五味子醇甲、五味子醇乙、五味子丙素、五味子酯甲对损伤肝细胞的保护作用。 方法 采用过氧化氢（H2O2）诱导HL-7702肝细胞损伤模型，在五味子甲素组中，将细胞随机分为正常组、模型组及1、5、25 μmol/L五味子甲素加药组，采用CCK-8法检测五味子类甲素对损伤HL-7702细胞增殖力（A值）的影响，采用自动生化分析仪检测五味子类甲素对损伤肝细胞释放谷草转氨酶（AST）的影响。其余五味子乙素、醇甲、醇乙、丙素、酯甲等组与甲素组操作相同。 結果 与各自的模型组比较，五味子甲素、醇甲3个浓度组A值及AST浓度差异无统计学意义（P > 0.05），五味子醇乙、乙素、丙素的1、5 μmol/L组及五味子酯甲25 μmol/L组A值显著提高（P < 0.05），AST浓度显著降低（P < 0.05）。 结论 不含有亚甲二氧基的五味子甲素、醇甲对损伤肝细胞无保护作用，含有亚甲二氧基的五味子醇乙、乙素、丙素和酯甲对损伤肝细胞具有一定的保护作用。

[关键词] 五味子；联苯环辛烯类木脂素；亚甲二氧基；活性基团；氧化损伤；保肝作用

[中图分类号] R286.55 [文献标识码] A [文章编号] 1673-7210（2017）07（a）-0035-04

[Abstract] Objective To evaluate the protective effects of deoxyschizandrin， schisandrin B， schisandrol A， schisandrol B， schisandrin C and schisantherin A on injured hepatocytes， respectively. Methods The H2O2 was used to induce model of injured HL-7702. In the deoxyschizandrin groups， the cells were randomly divided into normal group， model group and 1， 5， 25 μmol/L of deoxyschizandrin dosing group. The influence of deoxyschizandrin for the proliferation （A value） of injured HL-7702 was detected by cell counting kit-8， meanwhile， the influence of deoxyschizandrin for the aspartate aminotransferase （AST） released by the injured hepatocytes was detected by automatic biochemistry analyzer. The other groups of schisandrin B， schisandrol A， schisandrol B， schisandrin C and schisantherin A had the same operation with deoxyschizandrin. Results Compared with model groups， there were no significant differences of A values and AST concentration in the three concentration groups of deoxyschizandrin and schisandrol A （P > 0.05）， while the A values in 1， 5 μmol/L groups of schisandrol B， schisandrin B， schisandrin C and 25 μmol/L group of schisantherin A were increased significantly （P < 0.05）， the AST concentrations in which were decreased significantly （P < 0.05）. Conclusion Deoxyschizandrin and schisandrol A without any methylenedioxy have no protective effects for the injured hepatocytes， while schisandrol B， schisandrin B， schisandrin C and schisantherin A with the methylenedioxy have certain protective effects for the injured hepatocytes.

[Key words] Fructus Schisandrae Chinensis； Dibenzocyclooctadiene lignans； Methylenedioxy； Active group； Oxidative damage； Hepatoprotective effect

五味子类木脂素主要分布于五味子科[1]，是五味子科植物的特征性成分[2]。该科植物五味子[Schisandra chinensis（Turcz.）Baill.]和华中五味子（Schisandra sphenanthera Rehd. et Wils.）的干燥成熟果实分别作为“五味子”和“南五味子”入药[3]。20世纪70年代，我国学者发现五味子和南五味子具有明显的保肝降酶作用，其活性成分主要为五味子类木脂素[4]。目前研究较多的主要有五味子甲素、五味子乙素、五味子醇甲、五味子醇乙、五味子丙素和五味子酯甲[5]，其中五味子醇甲、醇乙、乙素和丙素为五味子的特征性成分[6]，五味子甲素和酯甲为南五味子的特征性成分[7]。

研究发现，五味子类木脂素保肝活性存在构效关系[8]，一般认为苯环上的亚甲二氧基是该类成分的重要活性基团[9-10]。2……