杜苏萌 王媛 任勇 李建鹏 王燕子

摘要 [目的]研究用大孔树脂吸附分离虎杖中白藜芦醇的特性及其稳定性。[方法]分析大孔树脂对白藜芦醇的吸附曲线，分析流速、乙醇体积分数、提取时间、提取温度、pH等因素对大孔树脂吸附白藜芦醇的影响。[结果]AB-8树脂对虎杖中白藜芦醇的吸附更接近单分子层吸附。以1.5 mL/min流速通过层析柱时吸附率更高，用70%乙醇提取虎杖中白藜芦醇时提取率最高，3 h是最佳提取时间；60 ℃是最佳提取温度；pH为4时白藜芦醇提取率最高。稳定性研究表明，白藜芦醇在酸性条件下保存浓度基本不变。[结论]用大孔树脂吸附分离白藜芦醇具有一定的优势。

关键词 虎杖；白藜芦醇；吸附；稳定性；大孔树脂

中图分类号 S567.23+6；Q599 文献标识码 A 文章编号 0517-6611（2017）13-0130-03

Research on the Adsorption Characteristics and Stability of Resveratrol from Polygonum cuspidatum

DU Su-meng， WANG Yuan， REN Yong et al

（Department of Environmental and Safety Engineering，Taiyuan Institute of Technology，Taiyuan，Shanxi 030008）

Abstract [Objective]The research aimed to study the adsorption characteristics and stability of resveratrol from Polygonum cuspidatum by macroporous resin adsorption.[Method]The adsorption curve of resveratrol was analyzed by macroporous resin.The effects of flow rate，ethanol volumn fraction，extraction time，extraction temperature and pH on the adsorption of resveratrol by macroporous resin were analyzed.[Result]The adsorption of AB-8 resin for resveratrol was more close to the monolayer adsorption.When the flow rate was 1.5 mL/min， the adsorption rate was higher； extraction rate of resveratrol from Polygonum cuspidatum with 70% ethanol was the highest；3 h was the optimum extraction time； 60 ℃ was the optimum extraction temperature； when the pH was 4， extraction rate of resveratrol was highest.Stability research showed that resveratrol concentration kept unchanged under acidic conditions.[Conclution]Adsorption and separation of resveratrol by macroporous resin has some advantages.

Key words Polygonum cuspidatum；Resveratrol；Adsorption；Stability；Macroporous resin

白藜蘆醇是植物受到外界刺激而产生的一种植物抗毒素[1-2]，1940年首次从毛叶藜芦根部分离得到[3-5]，目前已在虎杖、葡萄、花生等至少72种植物中发现白藜芦醇的存在[6-7]。研究表明，白藜芦醇具有多种药理活性[1-2]，可预防癌症的发生及发展，具有广泛的应用价值，市场需求量大。白藜芦醇的提取方法有微波辅助法、超声提取法、超临界CO2萃取法、酶法、有机溶剂提取法[8-10]。大孔树脂是20世纪60年代末发展起来的一类新型树脂[11]，具有物理化学性质稳定、吸附和交换容量大、比表面积大、吸附速度快、选择性好、吸附条件温和、再生容易、强度好等优点，广泛用于天然植物中各种活性成分的提取与纯化[12]。笔者研究AB-8树脂吸附葡萄和虎杖中白藜芦醇的吸附特性、吸附的影响因素，寻找AB-8大孔树脂吸附白藜芦醇的最优条件，为更好地提取和分离白藜芦醇提供一定的理论依据。……