孙枭琼 陈永亮 孙慎丽 陈玉倩 朱笛 周晓燕 高梦杰 韩庆典

摘要 [目的] 研究腐殖酸钠（HA-Na）对镉胁迫下冬小麦种子萌发及根系生长的影响。[方法]以鲁麦22号为试验材料，研究不同浓度Cd2+溶液处理对冬小麦种子萌发和根系生长的影响，以及不同浓度HA-Na溶液对Cd2+胁迫下冬小麦种子萌发和根系生长的影响。[结果] Cd2+胁迫使冬小麦种子萌发和根系生长均受到抑制，发芽率、发芽指数、活力指数、幼苗根长及芽长均降低。低浓度的HA-Na提高了Cd2+胁迫下冬小麦种子发芽势、发芽率、发芽指数、活力指数以及幼苗根长、芽长，部分缓解了Cd2+胁迫对冬小麦种子萌发和幼苗生长的抑制作用。其中以50 μmol/L HA-Na溶液处理的缓解效果最好，但随着HA-Na浓度的增大，其对Cd2+胁迫的缓解效应逐渐减弱，当HA-Na浓度达200 μmol/L时，会明显加剧Cd2+胁迫的毒害作用。[结论]该研究为被重金属污染的农田土壤治理和改良提供了理论依据。

关键词 冬小麦；腐殖酸钠；镉胁迫；萌发；生长

中图分类号 S145.4；S512.1+1 文献标识码 A 文章编号 0517-6611（2017）13-0034-02

Effect of Sodium Humate on Winter Wheat Seed Germination and Root Growth under Cadmium Stress

SUN Xiao-qiong， CHEN Yong-liang， SUN Shen-li，ZHOU Xiao-yan* et al

（College of Life Science，Linyi University，Linyi，Shandong 276005）

Abstract [Objective] To study the effect of sodium humate（HA-Na） on winter wheat seed germination and root growth under cadmium stress. [Method] With Lumai 22 as material，we analyzed effects of different concentrations of Cd2+ solution on winter wheat seed germination and root growth，then explored effects of different concentrations of HA-Na solution on seed germination and root growth under cadmium stress. [Result] Winter wheat seed germination and root growth were inhibited by Cd2+ stress，germination index，vigor index，root length and bud length were decreased. Low concentrations of HA-Na improved winter wheat seed germination rate， germination index and vigor index，also improved root length，bud length under Cd2+ stress，and partial relieved seed germination and seedling growth under Cd2+ stress. The ameliorative effect of 50 μmol/L HA-Na was best among them. But with the increasing of concentration of HA-Na， the ameliorative effect of Cd2+ stress weakened gradually.It would significantly increase the toxic effects of cadmium stress while HA-Na concentration reached 200 μmol/L.[Conclusion] The study provides the theory basis for farmland soil management and improvement that has been polluted with heavy metals.

Key words Winter wheat；Sodium humate；Cadmium stress；Germination；Growth

礦产资源的大量开发和使用，导致土壤重金属污染越来越严重。研究表明，植物中的重金属累积到一定浓度，就会抑制其生长发育[1-3]。重金属镉（Cd）是重要的金属污染物之一，其化学行为和生态效应相对复杂，毒性很强，且容易被植物吸收。我国农田的镉污染面积已达1.4×104 hm2 [4]，且面积正迅速增加，亟需一些经济有效、切实可行的方法来解决。

冬小麦是世界上主要的粮食作物，也是山东半岛地区主要的种植作物。目前关于重金属对冬小麦生长发育的影响及危害机制已有部分报道[5-8]。……