辽宁雏蝗不同地理种群染色体研究
2017-07-13许姝娟史红全韩亚鹏陈德来柳鹏飞
许姝娟 史红全 韩亚鹏 陈德来 柳鹏飞



摘要 [目的]比较辽宁雏蝗(Chorthippus liaoningensis Zheng)3个不同地理种群(长白山种群、敦化种群、漠河种群)的染色体核型及C带带型。[方法]染色体常规压片法制片,使用BSG法处理染色体C带显带。[结果]辽宁雏蝗3个不同地理种群具有相同性别决定机制XO型,相同染色体数目2n(♂)=17,NF=23。3个地理种群染色体组式相似,染色体核型相同都为“1C”核型。在C带带型方面,3个地理种群的C带带型都比较单一,都仅具有着丝粒带。在异染色质总含量方面三者存在着差异。[结论]基于3个地理种群染色体核型及C带带型的特点,说明3个不同地理种群的辽宁雏蝗为同一种,但因为地理位置不同,同种内不同地理种群染色体C带带型存在一定差异。
关键词 辽宁雏蝗;染色体;核型;C带;地域种群
中图分类号 Q969 文献标识码 A 文章编号 0517-6611(2017)13-0013-03
Chromosome Analysis in different Populations of Chorthippus liaoningensis Zheng in China
XU Shu-juan, SHI Hong-quan, HAN Ya-peng et al
(College of Life Sciences and Technology, Longdong University, University Provincial Key Laboratory for Protection and Utilization of Longdong Bio-resources in Gansu Province, Qingyang, Gansu 745000)
Abstract [Objective] The aim was to compare chromosomal karyotypes and C-banding karyotypes of Chorthippus liaoningensis Zheng in three geographic populations. [Method] BSG method was used to carry out the banding treatment. [Result] The three geographic populations had the same chromosomal karyotypes of “1C” type and the same sex-determining mechanism of XO and the same chromosome number 2n(♂)=17 with NF=23. In C-banding, the three geographic populations all had centromeric band with different heterochromatin content. [Conclusion] In view of the chromosome karyotypes and the characteristics of C-banding, the three geographic populations are the same specie. Because the distinct geographical environments, slight differences in C-banding karyotypes are apparent among the three populations.
Key words Chorthippus liaoningensis Zheng;Chromosome;Karyotype;C-banding;Geographic population
染色体是遗传物质基因的载体,其各种特性包括染色体数目、形态及分裂过程的状态都有物种的特异性,可以用染色体确定物种的分类地位及物种与物种之间的亲缘关系。目前,染色体核型及C带带型研究在确定物种分类地位及亲缘关系方面得到了广泛的应用[1-3]。染色体C带带型可用作属级分类阶元的比较分析[4],也可用作种间关系的对比[5],还可用作种内水平种群亲缘关系和杂交带的种群细胞遗传的研究。Bugrov等[6]研究了日本北海道Podisma sapporensis 的2个染色体种群的C带核型。Shaw等[7]分析比较了Caledia captiva的4个染色体种群的C带核型,并对其种群细胞遗传进行了研究。马恩波等[8]比较分析了不同地域种群山稻蝗染色体C带核型。……
