徐淳 李润平

[摘要] 目的 考察間歇吸氧对小鼠肺、脑组织中抗氧化蛋白Peroxiredoxin 6（Prx 6）含量的影响，探讨间歇吸氧对抗氧中毒的机制。 方法 将小鼠随机分为正常组、持续暴露后即刻组、间歇暴露后即刻组、持续暴露后24 h组和间歇暴露后24 h组，通过不同暴露方法，分别取小鼠肺、脑组织采用免疫组织化学和Wester blot等方法测定Prx 6的含量。 结果 经高压氧暴露的各组小鼠肺组织中Prx 6的含量均明显高于正常组（P < 0.05或P < 0.01），持续暴露后即刻组与间歇暴露后即刻组差异无统计学意义（P > 0.05），间歇暴露后24 h组含量明显高于持续暴露后24 h组（P < 0.01），并且高于各自对应的暴露后即刻检查的结果（P < 0.01）。经高压氧暴露的各组小鼠脑组织中Prx 6的含量均明显低于正常组（P < 0.01），持续暴露后即刻组与间歇暴露后即刻组差异无统计学意义（P > 0.05），间歇暴露后24 h组与持续暴露后24 h组高于各自对应的暴露后即刻检查的结果（P < 0.05），且间歇暴露后24 h组含量接近正常组（P > 0.05）。 结论 间歇吸氧可以促进肺、脑组织中Prx 6的含量增加，从而增强组织的抗氧化能力。

[关键词] Peroxiredoxin 6；高压氧；间歇吸氧；氧中毒

[中图分类号] R459.6 [文献标识码] A [文章编号] 1673-7210（2016）02（c）-0018-04

Effects of intermittent hyperbaric oxygen exposure on the contents of peroxiredoxin 6 in the lung and brain of mice

XU Chun1 LI Runping2▲

1.Department of Outpatient， the 61565 Troops of People's Liberation Army， Beijing 102699， China； 2.Department of Diving Medicine， Faculty of Naval Medicine， the Second Military Medical University， Shanghai 200433， China

[Abstract] Objective To investigate the effects of intermittent hyperbaric oxygen exposure on the contents of peroxiredoxin 6 （Prx 6） in the lung and brain of mice， and to explore the mechanism underlying intermittent hyperbaric oxygen exposure protecting against oxygen toxicity. Methods Mice were randomly divided into normal group， immediate group after continuous exposure， immediate group after intermittent exposure， live 24 h group after continuous exposure， live 24 h group after intermittent exposure. The content of Prx 6 in the lung and brain were detected using immunohistochemistry and western blot analysis respectively. Results In lung， compared with normal group， Prx 6 contents of all mice experiencing hyperbaric oxygen exposure were increased （P < 0.05 or P < 0.01）. There was no significant difference between immediate group after continuous exposure and immediate group after intermittent exposure （P > 0.05）. The Prx 6 content of live 24 h group after intermittent exposure was significantly higher than that of live 24 h group after continuous exposure （P < 0.01）， and the result in each live 24 h group was also higher than that in each immediate group （P < 0.01）. In brain， compared with normal group， Prx 6 contents of all mice experiencing hyperbaric oxygen exposure were decreased （P < 0.01）. There was no significant difference between immediate group after continuous exposure and immediate group after intermittent exposure （P > 0.05）. The Prx 6 contents in each live 24 h group was higher than that in each immediate group （P < 0.05）， and the content of live 24 h group after intermittent exposure was close to the normal group （P > 0.05）. Conclusion Intermittent hyperbaric oxygen exposure can facilitate the expression of Prx 6 in lung and brain， and this may be related to the enhanced ability to protect against oxygen toxicity.

[Key words] Peroxiredoxin 6； Hyperbaric oxygen； Intermittent hyperbaric oxygen exposure； Oxygen toxicity

近年来发现过氧化物酶家族成员中的抗氧化蛋白（peroxiredoxin，Prxs）在体内广泛分布，现已知在哺乳动物中至少存在6种Prx亚型（Prx Ⅰ～Ⅵ）。它们利用包含巯基的蛋白，将过氧化物还原，起到降解毒性的作用[1-5]。高压氧暴露时，体内各种产生氧自由基的环节被激活，生成大量的活性氧（ROS），这些活性氧一方面可以氧化体内各种物质而导致组织损伤，另一方面也可以启动和参与细胞内多种物质代谢和信号转导过程，具有强大的病理生理学功能[6-10]。间歇吸氧很可能通过激活Prxs，进而降解活性氧，实现其对抗氧中毒发生的作用。故本研究将探讨间歇高压氧暴露对小鼠肺、脑组织中抗氧化蛋白（Prx 6）含量的影响，以期为阐明间歇吸氧预防氧中毒的作用机制提供部分科学依据。……