梁易晓，刘丽娜，秦顺义，王　昕，唐晓娟，赵方红

（天津农学院动物科学与动物医学学院，天津西青300384）

羧甲基壳聚糖硒对小鼠生长性能、血液硒含量及抗氧化功能的影响

梁易晓，刘丽娜，秦顺义*，王昕，唐晓娟，赵方红

（天津农学院动物科学与动物医学学院，天津西青300384）

为研究羧甲基壳聚糖硒对小鼠生长性能、血液硒含量及抗氧化功能的影响，本试验将120只KM小鼠（雌雄各半），随机均分为C组、TI组和T2组，分别喂基础日粮、添加亚硒酸钠的基础日粮和添加羧甲基壳聚糖硒的基础日粮，试验持续28 d。结果表明：T2组小鼠采食量和料重比较C组和T1组分别降低了5.24%、8.66%和4.27%、4.29%；但T2组、T1组和C组间小鼠的采食量、料重比及增重均无显著差异（P＞0.05）。T2组小鼠全血硒含量和血浆总抗氧化能力（T-AOC），较C组和T1组分别提高了78.95%（P＜0.01）、17.50%（P＜0.01）和17.24%（P＜0.05）8.05%（P＜0.05）；T1组小鼠全血硒含量和血浆T-AOC较C组分别提高了52.63%（P＜0.05）和8.75%（P＜0.05）。T2组和T1组小鼠全血谷胱甘肽过氧化物酶 （GSH-Px）活性分别较C组提高了24.65%（P＜0.01）和14.13%（P＜0.05）；T2组小鼠全血GSH-Px活性较T1组提高了9.22%，但未达差异显著水平（P＞0.05）。T2组小鼠血浆丙二醛（MDA）含量分别较C组和T1组降低了29.40%（P＜0.01）和18.66%（P＜0.05）；T1组小鼠血浆MDA含量较C组降低了13.21%（P＜0.05）。结果提示：羧甲基壳聚糖硒能够提高小鼠血液硒含量和抗氧化能力，其效果优于亚硒酸钠；羧甲基壳聚糖硒对小鼠生长性能无影响。

羧甲基壳聚糖硒；小鼠；生长性能；硒含量；抗氧化功能

［Abstract］This experiment was conducted to determine the effects of carboxymethyl chitosan selenium on growth performance，blood selenium content and antioxidation capacity in mice.One hundred and twenty KM mice，female and male in half，were randomly assigned into three dietary treatment groups for a 28 days study，which were fed basal diets，basal diets supplemented with sodium selenite and basal diets supplemented with carboxymethyl chitosan selenium，respectively. The results showed that feed intake and feed/gain ratio（F/G）in T2 group were 5.24%，8.66%and 4.27%，4.29%lower than those in T1 group and C group，while there were no significant differences in feed intake，F/G and weight gains of mice among T2 group，T1 group and C group（P＞0.05）.Blood selenium contents and plasma total antioxidant capacity（T-AOC）levels in T2 group were 78.95%（P＜0.01），17.50%（P＜0.01）and 17.24%（P＜0.05），8.05%（P＜0.05）higher than those in C group and T1 group，blood selenium contents and plasma T-AOC levels in T1group were 52.63%（P＜0.05）and 8.75%（P＜0.05）higher than those of C group.Blood glutathione peroxidase（GSH-Px）activities in T2 group and T1 group were 24.65%（P＜0.01）and 14.13%（P＜0.05）higher than that of C group.Blood GSH-Px activities in T2 group were 9.22%higher than that in T1 group（P＞0.05）.Plasma malonaldehyde（MDA）contents in T2 group were 29.40%（P＜0.01）and 18.66%（P＜0.05）lower than that in C group and T1 group.Plasma MDA contents in T1 group were 13.21%（P ＜0.05）lower than that in C group.The results indicated that carboxymethyl-chitosan selenium supplementation could increase blood selenium content and antioxidant capacity in mice，and its effects were better than sodium selenite in mice. While carboxymethyl-chitosan selenium had no effect on growth performance in mice.

［Key words］carboxymethyl-chitosan selenium；mice；growth performance；selenium content；antioxidation capacity

大量研究表明，微量元素硒具有抗氧化、促生长、提高免疫力等多种生物学功能。饲料中添加的硒包括有机硒和无机硒两大类，与无机硒相比，有机硒具有毒性低、吸收利用率高、适口性好等特点。……