杜 娟，张德芹，文 静，黄春丽，张青霞，周文龙，刘 花

（天津中医药大学中医药研究院，天津 300193）

Abstract:［Objective］To observe the effects of Huangdi Jixian decoction guided by “reinforcing and reducing，adjustment the balance”method on the FBG and HbA1c in type 2 diabetic rats.［Methods］Sixty male SD rats of clean grade，randomly selected 10 rats as the normal control group，the remaining 50 of given high fat diet add disposable small dose of intraperitoneal injection of STZ add stress to induce rat model of type 2 diabetes mellitus.After the success of modeling，50 rats were randomly divided into model group，western medicine group，Chinese medicine treatment of low，medium and high dose groups，10 rats in each group.All groups were treated 8 weeks，before and after administration of the body weight，the determination of FPG，HbA1c were administered.［Results］Western medicine treatment group，Chinese herbal medicine in high，medium and low dose group rats were improved in general condition after treatment.The weight in the high dosage group of Chinese medicine increased significantly than those in the other four groups（P＜0.05）.The FBG in the western medicine treatment group，Chinese medicine of high，middle dose group were decreased significantly than model group（P＜0.05 or 0.01）.Traditional Chinese medicine of high dose can significantly reduced HbA1c.［Conclusion］Huangdi Jixian decoction can obviously improve the diabetic rats in general condition，significantly reduced blood glucose and HbA1c.
Key words:Type 2 diabetes；Huangdi Jixian decoction；FBG；HbA1c

升清降浊方对MSG肥胖大鼠胰岛素增敏作用的影响*

杜 娟，张德芹，文 静，黄春丽，张青霞，周文龙，刘 花

（天津中医药大学中医药研究院，天津 300193）

［目的］研究升清降浊方（SQJZF）对L-谷氨酸钠（MSG）肥胖大鼠胰岛素增敏作用的影响及其机制研究。［方法］复制MSG肥胖大鼠模型，随机分为模型对照组、阳性药罗格列酮组、阳性药非诺贝特组、SQJZF高剂量组（含生药6 g/kg）、SQJZF中剂量组（含生药3 g/kg）、SQJZF低剂量组（含生药1.5 g/kg），另取正常组大鼠8只。连续给药50 d后，分别检测大鼠血清瘦素和肌糖元含量；实时荧光定量聚合酶链式反应（PCR）检测药物对MSG大鼠骨骼肌过氧化物酶体增殖物激活受体（PPAR）、瘦素（Leptin）、胰岛素受体底物-1（IRS-1）、胰岛素受体底物-2（IRS-2）、葡萄糖转运蛋白4（GLUT4）的基因表达水平。［结果］模型组MSG大鼠肌糖元和血清瘦素含量显著升高（P＜0.01或P＜0.05），相关基因PPAR、Leptin、IRS-1、IRS-2、GLUT4的相对表达显著降低（P＜0.01）；给药50 d后，各给药组肌糖元和血清胰岛素含量均有降低，对相关基因的相对表达均有一定的调节作用。［结论］升清降浊方可能通过激活PPAR受体，调控Leptin基因的表达，增加胰岛素的敏感性。

升清降浊方；MSG肥胖大鼠；胰岛素抵抗

升清降浊方（SQJZF）来源于临床经验方，由桑叶、荷叶、丹参等中药组成，具有升清降浊，化瘀消痞的功效，是治疗糖尿病前期糖脂代谢紊乱的有效方剂。已有实验研究表明，升清降浊方对自发性2型糖尿病模型KK-Ay和实验性2型糖尿病模型小鼠有降低血糖、调节血脂的作用［1-3］，对四氧嘧啶模型大鼠有降低血糖、调节血脂的作用［4］，对高脂血症家兔有降低血脂，提高脂解酶活性的作用［5-6］。……