杨丽娜，罗志红，栾晓文，宁钰清，李富强，宁亚功

（成都军区昆明总医院附属中医医院，昆明 650032）

Abstract:［Objective］To investigate the effect of simvastatin on atherosclerotic plaque instability model of thrombosis in rabbits.［Methods］Male New Zealand rabbits were randomly divided into 2 groups.One group（sham group）was always given a normal diet.Another group was fed high-fat diet for four weeks，and then abdominal aortic endothelium was damaged by using 4F Forgarty catheter.The rabbits were replaced for normal diet until being sacrificed，and were randomly divided into model group and simvastatin group.Simvastatin group received simvastatin ［10 mg/（kg·d）］for 4 weeks.Sham group and model group were given an equal volume of normal saline.Rabbits were sacrificed after 4 weeks of drug intervention in each group.Rabbits plaque rupture were triggered by giving intraperitoneal injections of venom under（0.15 mg/kg）and the ear vein injection of histamine（0.02 mg/kg）in 48 h and 24 h twice before sacrifice.Rabbits abdominal aortic thrombosis were observed in the stereo microscope after sacrifice.Rabbits were detected vWF and D-Dimer by ELISA，and were detected concentration of TXB2 and 6-keto-PGF1α by radioimmunoassay，and were calculated the ratio of TXB2/6-keto-PGF1α.［Results］After four weeks of treatment with simvastatin，simvastatin group were observed to reduce plaque site thrombosis under stereo microscope，and there was a significant difference compared with the model group（P＜0.01）.Plasma levels of vWF：Ag and D-Dimer were lower in the simvastatin group，and there was a significant difference compared with the model group（P＜0.01）.Plasma concentration of TXB2 was lower and plasma concentration of 6-keto-PGF1α was higher，and there was a significant difference compared with the model group（P＜0.05）.The ratio of TXB2/6-keto-PGF1α was lower，and there was a significant difference compared with the model group（P＜0.01）.［Conclusion］Simvastatin can repair damaged endothelial cells and improve blood hypercoagulable state，inhibition of platelet aggregation，and thus play a role in anti-thrombosis.This study provides experimental evidence that simvastatin prevents thrombosis in the clinical for patients with AS unstabled plaque.
Key words:simvastatin；atherosclerosis；plaque instability；thrombosis

黄地吉仙汤对2型糖尿病大鼠FBG及HbA1c影响的实验研究*

杨丽娜，罗志红，栾晓文，宁钰清，李富强，宁亚功

（成都军区昆明总医院附属中医医院，昆明 650032）

［目的］观察“补泻同施，调节平衡”法指导组方的经验方黄地吉仙汤对2型糖尿病大鼠空腹血糖（FBG）、糖化血红蛋白（HbA1c）的影响。［方法］将清洁级雄性SD大鼠60只，随机抽取10只设置为空白对照组，其余50只给予高脂高糖饲料加一次性小剂量腹腔注射链脲菌素（STZ）与精神紧张法诱发2型糖尿病大鼠模型。造模成功后随机分为模型组、西药治疗组、中药治疗低、中、高剂量组，每组10只。除空白对照组外，其余5组分别喂药观察8周，给药前后观察大鼠一般情况、测定体质量、FBG、HbA1c指标。［结果］西药治疗组、中药高、中、低剂量组大鼠经给药治疗后，一般情况均有不同程度的改善，中药高剂量组体质量与其他4组比较明显升高（P＜0.05）；西药治疗组、中药高、中剂量组与模型组比较血糖明显减低（P＜0.05）；中药高剂量组具有明显降低HbA1c的作用。［结论］黄地吉仙汤可以明显改善糖尿病大鼠的一般情况，对糖尿病大鼠的体质量减轻具有缓解作用，能显著降低血糖及HbA1c，有效治疗2型糖尿病。

2型糖尿病；黄地吉仙汤；空腹血糖；糖化血红蛋白

糖尿病（DM）是由于胰岛素分泌不足或胰岛素作用低下而引起的一种代谢性疾病，以慢性高血糖为主要特征，是仅次于心血管疾病和恶性肿瘤的致死性疾病［1］。……