刘坦河南省洛阳市郑州大学附属洛阳中心医院检验科，河南洛阳471009

酶联免疫法在结核病检测中重要价值

刘坦
河南省洛阳市郑州大学附属洛阳中心医院检验科，河南洛阳471009

目的分析结核感染酶联免疫法在结核病检测中的重要价值。方法选取该院2013年3月—2014年5月收治的62例疑似结核病患者作为对象进行研究，对其实施结核感染酶联免疫法试验与结核菌素试验，记录两种检测方法结果，并比较两种方法特异度、敏感度、假阳性率、假阴性率、阳性似然比（LR+）及阴性似然比（LR-）。结果结核菌素试验结果显示阴性25例，阳性37例，结核分歧杆菌培养阴性24例，阳性38例；结核感染酶联免疫法结果显示阴性16例，阳性46例，结核分歧杆菌培养阴性13例，阳性49例；结核感染酶联免疫法特异度、灵敏度均显著优于结核菌素试验，差异有统计学意义（P＜0.05）；结核感染酶联免疫法诊断阳性似然比（LR+）为11.94、阴性似然比（LR-）为0.99。结论结核感染酶联免疫法检测敏感性更高，且检测即时性好，适用于结核病检测，临床价值较高。

结核感染酶联免疫法；结核菌素试验；结核病

［Abstract］Objective To analyze the important value of tuberculosis infection euzymelinked immunosorbent assay in de‐tecting bacillary phthisis.M ethods 62 cases of patients suspected with bacillary phthisis admitted and treated in our hospi‐tal from March 2013 to May 2014 were selected as the research objectes,and were given tuberculosis infection eu‐zymelinked immunosorbent assay test and tubercalin test,the detection method results were recorded,and the specificity, sensitivity,false positive rate,false negative rate,positive likelihood ratio and negative likelihood ratio were compared be‐tween the twomethods.Results The tubercalin test results showed that 25 cases were negative,37 cases were positive,24 negative cases and 38 positive caseswere cultivated bymycobacterium tuberculosis,the tuberculosis infection euzymelinked immunosorbent assaymethod results showed that 16 caseswere negative and 46 caseswere positive,and 13 negative cases and 49 positive cases were cultivated bymycobacterium tuberculosis,and the specificity and sensitivity of tuberculosis in‐fection euzymelinked immunosorbent assay were obviously better than those of tubercalin test,and the difference has statis‐tical significance,P《0.05,the diagnostic positive likelihood ratio and negative likelihood ratio of tuberculosis infection eu‐zymelinked immunosorbent assay were respectively 11.94 and 0.99.Conclusion The detection sensitivity of tuberculosis in‐fection euzymelinked immunosorbent assay is higher,and the detection instantaneity is better,and it is suitable for bacillary phthisis detection with higher clinical value.

［Key words］Tuberculosis infection euzymelinked immunosorbent assay;Tubercalin test;Bacillary phthisis

尽管结核疫苗的应用及有效药物的控制使其有一定缓解趋势，但由于近年来人口大量流动，随着人类免疫缺陷病毒及结核分歧杆菌耐药菌株感染率上升，结核病死率逐渐升高[1]。快速准确检测为控制结核病的关键，因此高效、准确的检测方法显得极为重要，传统结核病检测方法效果均不佳[2]。目前，结核感染酶联免疫法准确率较高、即时性较好，包涵性较强，结果受致病性非结核分歧杆菌干扰较小，同时不受接种卡介苗影响，检测过程操作简单，不受检测人员影响[3-4]。……