张　扬(连云港市赣榆区人民医院药剂科，江苏 连云港　222100)



复方中药组方对高尿酸血症影响的研究

张扬*(连云港市赣榆区人民医院药剂科，江苏 连云港222100)

DOI10.14009/j.issn.1672-2124.2016.03.029

摘要目的：研究复方中药组方(AG)对高尿酸血症的治疗作用。方法：分别用酵母，氧嗪酸钾盐，腺嘌呤和乙胺丁醇三种方法诱导高尿酸血症模型，给药后通过检测尿酸(UA)、黄嘌呤氧化酶(XOD)、腺苷脱氨酶(ADA)等指标，探讨AG的治疗作用。结果：AG各剂量组均可显著降低酵母所致小鼠高尿酸血症的血清UA值，差异有统计学意义(P<0.05)；AG高剂量组可显著降低氧嗪酸钾盐所致小鼠高尿酸血症的血清UA值，差异有统计学意义(P<0.05)；AG各剂量组在7、14、21 d均可极显著的降低腺嘌呤和乙胺丁醇大鼠高尿酸血症的血清UA值，差异有统计学意义(P<0.01)；在此模型中，7、14 d 时AG高、中剂量组小鼠的尿液UA值显著降低，低剂量组小鼠尿液UA值在7 d有显著的降低，差异均有统计学意义(P<0.05)；AG各剂量组的血清XOD活力均有极显著的降低，差异均有统计学意义(P<0.01)。结论：AG对不同造模法所致的高尿酸血症均有减低体内血清UA含量，抑制UA生成的作用。

关键词复方中药组方； 高尿酸血症； 尿酸

Research on Effects of Chinese Medicines Section(AG) on Hyperuricemia

ZHANG Yang(Dept.of Pharmacy, Lianyungang Ganyu District People’s Hospital， Jiangsu Lianyungang 222100, China)

ABSTRACTOBJECTIVE：To research the treatment effects of Chinese medicines section(AG) on hyperuricemia. METHODS: Three methods interms of yeast, oxonic acid potassium salt, adenine and ethambutol were used to induce hyperuricaemia model; the treatment effects of AG were investigated through the detection of uric acid(UA), xanthine oxidase(XOD) and adenosine deaminase(ADA) and some other indexes after administration. RESULTS: Different dose groups of AG could significantly decrease the serum UA value in the hyperuricemia mice which induced by yeasts, the difference was statistically significant(P<0.05). The high dose group of AG could significantly decrease the serum UA value in the hyperuricemia mice which induced by oxonic acid potassium salt, with statistical significance(P<0.05). Different dose groups of AG could significantly decrease the serum UA value in the hyperuricemia rat induced by adenine and ethambutol in 7 d, 14 d and 21 d, with statistically significant difference(P<0.01). In this model, high and middle dose group of AG could significantly decrease the level of urine UA value in 7 d and 14 d, and low dose group of AG could significantly decrease the level of urine UA value in 7 d, with statistically significant difference(P<0.05)；Different dose groups of AG could significantly decrease the serum XOD activity , the difference was statistically significant(P<0.01). CONCLUSIONS: AG could significantly decrease the serum UA value in different models induced by hyperuricemia and inhibit the generation of UA.

KEYWORDSChinese medicines section; Hyperuricemia; Uric acid

痛风是一种以持续性高尿酸血症导致尿酸钠晶体在关节及其周围组织沉积为特征的嘌呤代谢性疾病[1]。血尿酸(UA)升高是痛风、高尿酸血症的重要生化指标[2-3]，高尿酸血症被认为是发生痛风的前期状态。近年来，高尿酸血症和痛风的发病率日益增高，人们越来越重视药物治疗和新药地研发。目前，治疗高尿酸血症的药物主要包括抑制UA生成的药物，如別嘌呤醇；促进UA排泄的药物，如苯溴马隆；尿酸酶类药物，如拉布立酶等[4-5]。连云港市赣榆区人民医院(以下简称“我院”)根据传统中医配方研制出复方中药组方(AG)，经初步筛选发现疗效较好，本研究通过药理研究手段探讨AG对实验动物高尿酸血症的治疗作用及影响机制，现报告如下。

1资料和方法

1.1实验动物

清洁级小鼠140只(18～22 g)，雄性，由扬州大学比较医学中心提供，许可证号：SCXK苏2014-0007；清洁级大鼠80只(180～220 g)，雄性，由浙江省实验动物中心提供，许可证号：SCXK浙2014-0008。……