严锦，程思杰，余灵祥，洪智贤，吴建中，王瑞兰，张克明，孙百军，张培瑞，王兆海，杨鹏辉，张绍庚（中国人民解放军第三〇二医院　肝胆外科中心，北京　100039）



肝脏肿瘤标本资源库的建立、维护及管理

严锦，程思杰，余灵祥，洪智贤，吴建中，王瑞兰，张克明，孙百军，张培瑞，王兆海，杨鹏辉，张绍庚
（中国人民解放军第三〇二医院肝胆外科中心，北京100039）

[摘 要]目的探讨建立以肝癌组织样本为主的肝脏外科生物样本库及相关数据信息化管理的方法和标准化操作规程，为临床与基础研究提供高质量的临床标本资源平台。方法 收集肝脏肿瘤手术患者的血液标本（血清、血浆、白细胞）、组织标本(癌、癌旁、远端正常组织)，将处理好的血液标本于-80 ℃冰箱保存，新鲜组织置液氮保存，定期随机抽取液氮保存的肿瘤组织样本进行RNA、DNA的提取及鉴定。结果现收集并保存有完整临床信息及随访资料的肝脏肿瘤患者血液标本22 103份，组织标本3 319例，其中肝脏肿瘤组织标本807例，包括肝脏恶性肿瘤762例，良性肿瘤23例，肝硬化组织22例。随机抽取组织RNA、DNA鉴定均符合标本质量标准，能够满足后续实验要求。结论 初步建立标准化、规范化的肝脏肿瘤标本库，制定了标准化的标本库维护和管理规程，为肝脏肿瘤基础和临床研究提供了宝贵的实验材料。

[关键词]肝脏肿瘤；生物样本库

Establishment,maintenance and management of liver tumor biobank YAN Jin,CHENG Si-jie,YU Ling-xiang,

HONG Zhi-xian,WU Jian-zhong,WANG Rui-lan,ZHANG Ke-ming,SUN Bai-jun,ZHANG Pei-rui,WANG Zhao-hai,YANG Peng-hui,Zhang Shao-geng.Center of Hepatobiliary Surgery,302 Military Hospital,Beijing 100039,China

Abstract objectiveTo study the measures and standard operation procedure for establishing the biobank of liver surgical samples and information management of related data about samples,providing a high quality clinical specimen resource platform for clinical and basic research.MethodsBlood samples from patients with liver tumor(serum,plasma,leukocyte)and tissue samples(cancer tissues,tissues adjacent to carcinoma and the distal normal tissues)were collected,and then be kept in -80 ℃ refrigerator or in liquid nitrogen for preservation.Samples were randomly selected for the extraction and identification of RNA and DNA.ResultsA total of 22 103 blood samples with complete clinical information and follow-up data of liver tumor patients were collected and saved,807 cases of tissue specimens,of which there were 762 cases of malignant liver tumor,23 cases of benign tumors,22 cases of liver cirrhosis.Random tissue specimens of RNA and DNA identifications were preserved in high quality standards and could meet the requirements of the following studies.Conclusion A standardized and normalized liver tumor specimen biobank is established and the standard specimen library maintenance and management procedures are formulated,which provides valuable experimental materials for basic and clinical research for liver tumor.

Key words liver tumor; biobank

肝细胞癌（hepatocellular carcinoma，简称肝癌）是全球范围内最常见的恶性肿瘤之一，位居全球恶性肿瘤发病率第3位，死亡率第2位[1]。近年来肝癌在世界范围内发病率仍呈现上升趋势，尤其在东南亚国家肝癌的高患病率和高病死率（总体病死率0.95），已成为当前社会主要的健康威胁之一[2]。……