迪丽阿热姆·尼加提， 阿吉艾克拜尔·艾萨

(1新疆医科大学药学院；2中国科学院新疆理化技术研究所干旱区植物资源化学重点实验室， 乌鲁木齐　830011)

HPLC法测定阿图什无花果叶汁中3种香豆素的含量

迪丽阿热姆·尼加提1,2， 阿吉艾克拜尔·艾萨1,2

(1新疆医科大学药学院；2中国科学院新疆理化技术研究所干旱区植物资源化学重点实验室， 乌鲁木齐830011)

摘要：目的高效液相色谱法(HPLC法)测定阿图什无花果叶汁中伞形花内酯、佛手柑内酯和补骨脂素3种香豆素的含量。方法色谱柱Sunfire C18ODS (250 mm×4.6 mm,5 μm)，流速1 mL/min，检测波长为220 nm，柱温30℃，流动相为乙腈-水，流动相配比为0~10 min，乙腈25%；10~12 min，乙腈由25%梯度增至50%；12~20 min，乙腈50%；20~22 min，乙腈由50%梯度增至100%。结果伞形花内酯、佛手柑内酯和补骨脂素分别在0.001~0.019 mg/mL(R2=0.999 9)、0.01~0.19 mg/mL(R2=0.999 7)和0.01~0.19 mg/mL(R2=0.999 9)范围内与峰面积呈良好线性关系，平均回收率分别为98.09%(RSD=0.85%)、102.07% (RSD=1.07%)和101.00%(RSD=0.71%)。阿图什无花果叶汁中伞形花内酯、佛手柑内酯和补骨脂素3种香豆素的含量分别为1.09、9.89、24.58 mg/g。结论HPLC法简便、准确、稳定，可用于测定无花果叶汁中伞形花内酯、佛手柑内酯和补骨脂素的含量。

关键词：无花果叶汁； 香豆素； 高效液相色谱法

Determination of three coumarins in Ficuscarica L. Latex by HPLC methods

Dilaram Nijat1,2, Hajiakber Aisa1,2

(1SchoolofPharmacy,XinjiangMedicalUniversity;2KeyLaboratoryofPlantResources

andChemistryinAridRegions,XinjiangTechnicalInstituteofPhysicsandChemistry,

ChineseAcademyofSciences,Urumqi, 830011,China)

Abstract:ObjectiveTo determine the contents of Umbelliferone, Bergapten and Psoralen in Ficuscarica Latex by HPLC method. MethodsHPLC method conditions and procedure: Sunfire C18ODS column (250 mm×4.6 mm, 5 μm), with the flow rate of 1.0 mL/min, detectional wavelength at 220 nm under column temperature 30℃; acetonitrile-H2O as the mobile phase, 0~10 min, acetonitrile as 25%; afeter 10~12 min, acetonitrile as going up to 25%~50% gradually; 12~20 min, acetonitrile as 50%; 20~22 min, acetonitrile as going up to 50%~100% gradually. ResultsThere was a good linear relationship over the concentration range of (0.001~0.019) mg/ml, R2=0.999 9 for Umbelliferone, (0.01~0.19) mg/ml, R2=0.9997 for Bergapten and (0.01~0.19) mg/ml, R2=0.9999 for Psoralen. The average recovery rates of Umbelliferone, Bergapten and Psoralen were 98.09% (RSD=0.85%), 102.07% (RSD=1.07%) and 101.00% (RSD=0.71%), respectively. The contents of Umbelliferone, Bergapten and Psoralen were 1.09, 9.89 and 24.58 mg/g. ConclusionThe method is simple, accurate and stable, which could be applied to the determination of quality control.

Keyword:FicuscaricaL. Latex; Coumarins; HPLC

无花果，英文名为Fig，拉丁名为(Ficuscarica L.)，别称映日果、奶浆果、蜜果，属桑科榕属落叶乔木或灌木。全株含白色乳汁，花隐于囊状总花托内，外观只见果而不见花，故名无花果[1]。无花果原产于阿拉伯，后传入叙利亚、土耳其、中国等国，在我国新疆、山东、广西、江苏、浙江、陕西等地栽培也较为广泛。《本草纲目》[2]载：无花果“味甘、平、无毒，治五痔、咽喉痛”。《中药大辞典》[3]载：无花果“健胃清肠、消肿解毒，治肠炎、痢疾、喉痛、疮疖疥癣”。……