荣光宏，马颖才

(青海省人民医院消化内科，青海 西宁 810007)



结肠癌中HCCR和Survivin的表达及临床意义

荣光宏，马颖才※

(青海省人民医院消化内科，青海 西宁 810007)

摘要目的探讨HCCR与Survivin在结肠癌组织中的表达及临床意义。方法选取30例正常结肠组织和75例结肠癌组织，利用免疫组化法检测HCCR和Survivin蛋白的表达，通过分析二者在正常结肠组织和结肠癌组织中的表达水平及其与临床表现的关系，了解其表达及临床意义。结果HCCR在正常组织阳性表达率为3.3%，结肠癌组织中阳性表达率为70.7%，两者比较差异有显著意义(χ2=38.893，P<0.05)，其阳性表达与分化程度、淋巴结转移情况、Dukes分期有关(P<0.05)。Survivin在30例正常组织均呈阴性表达，在结肠癌组织中的阳性表达率为78.7%，两者比较差异有显著意义(χ2=53.870，P=0.000)，其阳性表达与分化程度、淋巴结转移情况、Dukes分期有关(P<0.05)。HCCR在癌组织中与Survivin的表达呈显著正相关(γs=0.308，P=0.007)。结论HCCR和Survivin在结肠癌组织中表达异常，其与癌组织的发生、发展密切相关。

关键词结肠肿瘤HCCRSurvivin免疫组化

EXPRESSION AND PROGNOSTIC SIGNIFICANCE OF

HCCR AND SURVIVIN IN COLORECTAL CANCER

Rong Guanghong，Ma Yingcai※

(Digestive Department of Qinghai Provincial People's Hospital，Xining，810007)

Abstract ObjectiveTo explore the expression of HCCR and Survivin in colorectal carcinoma tissues as well as their clinical prognostic significance.Methods The expression of HCCR and Survivin in 75 cases of colorectal carcinoma tissue and 30 cases of normal colorectal tissue were detected by SP immunohistochemistry method further investigate the relationship between the HCCR and Survivin expression level with colorectal carcinoma.Results There were 1 cases of HCCR positive express in 30 cases of normal colonial tissues，the positive rate of expression was 3.3%，there were 53 cases of HCCR positive express in 75 cases of colorectal carcinoma tissues，the positive rate of expression was70.7%，the difference was obvious(χ2=38.893，P<0.05).The positive expression of HCCR was correlated with differentiation grade，lymph node metastases and Dukes′stage(P<0.05).Survivin was expressed negatively in all of the normal colorectal tissue，the positive rate of which in colorectal carcinoma was 78.7%，the difference was obvious(χ2=53.870，P=0.000).The positive expression of Survivin was related to differentiation grade，lymph node metastases and Dukes′stage(P<0.05).The expression of HCCR was significant and positively correlated with that of Survivin in colorectal cancer(γs=0.308，P=0.007).Conclusion The deregulation of HCCR and Survivin expressions may correlate to the occurrence，development of colorectal carcinoma.The combined detection of HCCR and Survivin may have important significance for the determination of the biological behavior of colon cancer.

KeywordsColorectalHCCRSurvivinImmunohistochemistry

癌基因的激活是结肠癌发病的主要因素，通过对癌基因及其蛋白分子作用的研究，有助于我们了解结、直肠癌的发病机制[1-2]。人宫颈癌基因(HCCR)是参与人多种肿瘤发生、发展的基因[3-4]，是研究基因治疗恶性肿瘤的新靶点。Survivin(生存素)是抑制凋亡基因家族的成员，在多种人类肿瘤组织中表达，在肿瘤发生、发展中有重要的作用[5]。二者在结、直肠癌中表达及相互关系的报道结果不一。我们应用免疫组化技术检测HCCR和Survivin蛋白在正常结肠组织和结肠癌中的表达，进一步明确HCCR与Survivin在结肠癌组织中的表达及临床意义。

1资料与方法

1.1临床资料

收集青海省人民医院外科自2011年6月至2012年12月经病理学证实且接受手术治疗的结肠癌患者75例，所有病例术前均未行放疗、化疗及免疫治疗。其中男40例、女35例，年龄29～80岁；组织分化程度中高中分化腺癌27例、低分化腺癌48例；其中无淋巴结转移者49例，有淋巴结转移者26例；……