李瑞雪，洒玉萍，祁培宏，袁　明，曹志东

(1.青海大学医学院；2.青海省中医院肝病科)



穴位埋线对酒精性肝病大鼠的治疗作用

李瑞雪1，洒玉萍1，祁培宏2，袁明1，曹志东1

(1.青海大学医学院；2.青海省中医院肝病科)

摘要目的研究穴位埋线对大鼠肝功及血清相关指标的影响，探讨丰隆(双)、三阴交(双)穴位埋线治疗酒精性肝病的作用机制及其疗效。方法应用分子生物学方法检测穴位埋线对酒精性肝病大鼠的治疗作用。穴位埋线组采用丰隆(双)、三阴交(双)穴位埋线，空白组、模型组予蒸馏水灌胃，硫普罗宁组予硫普罗宁混悬液灌胃，埋线加硫普罗宁组埋线治疗同时辅以硫普罗宁混悬液灌胃，治疗4周后检测大鼠血清天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、肿瘤坏死因子(TNF-α)、IL-1β，内毒素(LPS)的含量。结果与空白组比较，模型组血清ALT、AST、TNF-α、IL-1β，LPS水平显著升高(P<0.01)；治疗组上述指标与模型组比较下降显著(P<0.05)。结论穴位埋线对大鼠肝功及血清相关指标存在影响，丰隆(双)、三阴交(双)穴位埋线能通过降低血清AST、ALT、TNF-α、IL-1β及内毒素水平对酒精性肝病发挥治疗作用。

关键词穴位埋线酒精性肝病大鼠

THERAPEUTIC EFFECT OF POINT EMBEDDING THERAPY

ON ALCOHOLIC LIVER DISEASE IN RATS

Li Ruixue1，Sa Yuping1，Qi Peihong2，Yuan Ming1，Cao Zhidong1

(1.Qinghai University Medical College；

2.Qinghai Province Hospital of traditional Chinese Medicine，Division of Liver Diseases，

Qinghai Xining 810001，China)

Abstract ObjectiveTo observe the effect of acupoint catgut embedding therapy on hepatic function and serum related indicators in rats，and investigate the function mechanism of acupoint catgut embedding therapy of “fenglong”and“sanyinjiao”in treating alcoholic liver disease(ALD).Methods The therapeutic effects of acupoint catgut embedding therapy in rats with alcoholic liver disease were detected by molecular biology methods.Acupoint catgut embedding therapy at “fenglong”and“sanyinjiao” was applied in the point embedding therapy group.Rats in the blank and model group were administrated distilled water.The tiopronin group was administration with gastric perfusion of tiopronin suspention.The point embedding therapy combined with tiopronin group was treated with acupoint catgut embedding therapy，at the same time supplemented by tiopronin suspention.After treatment of 4 weeks，some indices were detected in the serum such as ALT，AST，TNF-α，IL-1β，LPS in each group.Results Compared with the blank group，the serum ALT，AST，TNF-α，IL-1β，LPS in model group were markedly higher(P<0.01)；The ALT，AST，TNF-α，IL-1β，LPS in serum was significantly lower in therapy group than that in model group(P<0.05)．Conclusion Acupoint catgut embedding therapy has a definite effect on hepatic function and serum related indicators in rats.Acupoint catgut embedding therapy of“fenglong”and“sanyinjiao” exert the therapeutic effect by reducing ALT，AST，TNF-α，IL-1β，LPS levels in serum on alcoholic liver disease .

KeywordsAcupoint catgut embedding therapyAlcoholic liver diseaseRats

穴位埋线已广泛用于非酒精性脂肪肝的治疗，疗效独特。刘旭东、王甍[1-2]研究发现穴位埋线通过对肝脏组织胰岛素JNK1信号通路的调控发生作用。然而，关于穴位埋线治疗酒精性肝病的作用机制不明。本研究旨在观察丰隆(双)、三阴交(双)穴位埋线对酒精性肝病大鼠血清天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、肿瘤坏死因子(TNF-α)、IL-1β，内毒素(LPS)含量的影响，进一步探讨穴位埋线对酒精性肝病的作用机制。

1材料与方法

1.1动物及饲料

清洁级SD雄性大鼠75只，重量170~190 g，由甘肃中医学院科研实验中心提供(许可证号：SCXK甘2011-0001)。……