赵海龙，曹得萍，黄　欣，白海燕，李　伟，廖　博

(1.青海大学医学院病原生物学教研室；2.青海大学医学院实验诊断学教研室；

3.青海大学畜牧兽医科学院；4.重庆医科大学第二临床学院)



青南高原人体棘球蚴mtDNA基因多态性分析*

赵海龙1，曹得萍1，黄欣2，白海燕1，李伟3，廖博4

(1.青海大学医学院病原生物学教研室；2.青海大学医学院实验诊断学教研室；

3.青海大学畜牧兽医科学院；4.重庆医科大学第二临床学院)

摘要目的检测分析青南高原人体棘球蚴mtDNA基因型。方法对从青海玉树、果洛、海南、黄南藏族自治州收集的63个人体棘球蚴分离株，运用线粒体DNA的cox Ⅰ、nad Ⅰ、atp6 基因的特异性引物进行PCR扩增，对线粒体DNA特异目的片段进行序列分析鉴定。结果1)63个人体细粒棘球蚴分离株均为普通羊株(G1)；2)结果所得的9个基因型中有3个为青南高原地区所特有的基因型。结论本研究结果显示青南地区细粒棘球绦虫具有广泛性和普遍性的流行病学特征；同时青南高原又存在细粒棘球绦虫独特的核苷酸变异。

关键词青南地区高原人体棘球蚴基因多态性

ANALYSIS ON mtDNA POLYMORPHISM OF HUMAN

ECHINOCOCCOSIS IN SOUTHERN QINGHAI PLATEAU*

Zhao Hailong1，Cao Deping1，Huang Xin2，Bai Haiyan1，Li Wei3，Liao Bo4

(1.Department of pathogenic biology，Qinghai University Medical College，Xining 810001，China；

2.Department of experimental diagnostics，Qinghai University Medical College，Xining 810001，China；

3.Animal husbandry and Veterinary College of Qinghai University，Xining 810016，China；

4.The second clinical college of Chong Qing Medical University，401331，China)

Abstract ObjectiveDetection and analysis of the human Echinococcus mtDNA genotype in southern plateau of Qinghai Province.Methods The sample were collected fromYushu，Guoluo，Hainan and Huangnan Tibetan Autonomous Prefecture of Qinghai Province.63 cases of human hydatid isolated strains were amplified by PCR using primers specific for the mitochondrial DNA(mtDNA cox Ⅰ，nad Ⅰ，Atp6 )and the mitochondrial DNA specific fragment sequence was analysed and identificated.Results1)63 human Echinococcus granulosus isolates were common sheep strain(G1)；2)Among nine genotypes there were three specific genetypes in southern Qinghai Plateau.Conclusion The results of this study show that there have the general epidemiological characteristics of Echinococcus granulosus in south Qinghai area.At the same time，Echinococcus granulosus in southern Qinghai Plateau has its unique nucleotide variations.

KeywordsSouthern QinghaiPlateauHumanEchinococcosisGenetic polymorphism

在细粒棘球绦虫虫株鉴定和系统发生研究中，线粒体DNA(mtDNA)基因被广泛应用，而且mtDNA也用于检测细粒棘球绦虫属的遗传变异[1]。细粒棘球绦虫线粒体基因组为13588 bp，包括cox Ⅰ-cox Ⅲ基因，nad Ⅰ-nad Ⅵ基因和atp6基因[2，3]。将采自不同地区棘球蚴的mtDNA测序，得到10个基因型不同的地理株(G1-G10)，各虫株的宿主类别和地理分布存在差异[4]。

青海南部高原区位于昆仑山脉和唐古拉山脉之间，占青海省总面积的52%，属典型的高原大陆型气候，大部分地区海拔在4000～5000 m以上。……