赵　振，多　杰

(青海省人民医院呼吸科，西宁 810007)



移居高原不同时间健康人群血液HIF-1α、VEGF、EPO水平的变化及意义※

赵振，多杰*

(青海省人民医院呼吸科，西宁 810007)

摘要目的检测移居高原不同时间健康人血液HIF-1α、VEGF 、EPO水平的情况，并探讨其意义。方法选取移居高原(平均海拔4，200m)健康汉族120例及平原(平均海拔400m)健康汉族30例。分为移居高原1周组、1月组、1年组、5年组及平原组五组，每组各30例。采用ELISA 法及real-time PCR法分别检测各组血液中HIF-1α、VEGF、EPO的蛋白及mRNA表达水平，并行相关意义分析。结果移居汉族不同时间组HIF-1α、VEGF及EPO的表达均高于平原组(P<0.05)，随高原低氧暴露时间的逐渐延长移居汉族HIF-1α水平逐渐下降(P<0.05)，VEGF、EPO水平则随时间延长呈进行性升高(P<0.05)；HIF-1α、VEGF、EPO的mRNA表达水平与其血清蛋白表达水平的变化趋势一致。结论移居高原不同时间健康人群血液VEGF及EPO水平与HIF-1α的表达变化并不完全一致。

关键词高原时间HIF-1αVEGFEPO

EXPRESSIOINS AND SIGNIFICANTS OF BLOOD HIF-1α，

VEGF AND EPO IN HEALTHY PEOPLE IMMIGRATING TO HIGH

ALTITUDE AT DIFFERENT TIME※

Zhao Zhen，Duo Jie*

(Respiratory Department of Qinghai Provincial People′s Hospital，Xining，810007)

Abstract ObjectiveTo investigate the expressions of blood hypoxia inducible factor-1α(HIF-1α)，vascular endothelial growth factor(VEGF)and Erythropoietin(EPO)in healthy people who immigrating to high altitude at different time，and discusses their clinical significance.Methods 120 Han people immigrating to high altitude and 30 Han people in low altitude area were selected and divided into five groups，immigrating 1 week group，1 month group，1 year group，5 year group and low altitude group，with 30 persons in each group.HIF-1α，VEGF and EPO protein and mRNA levels were measured using ELISA and real-time PCR methods，and analyzed the related significance.Results The levels of HIF-1α，VEGF and EPO in people who immigrating to high altitude at different time were all higher than those in low altitude group.The levels of HIF-1α in Han populations immigrating to high altitude were decreased gradually with the exposure time(P<0.05).Levels of VEGF and EPO in immigrating Han populations were increased gradually with exposure time(P<0.05).HIF-1α，VEGF and EPO mRNA expression changed in a similar manner as their protein expressions.Conclusions The levels of blood VEGF and EPO in healthy people immigrating to high altitude at different time were not changed in a similar manner as the HIF-1α expression.

KeywordsHigh AltitudesDifferent TimeHIF-1αVEGFEPO

在机体的低氧调节适应过程中，低氧诱导因子1α(Hypoxia-inducible factor 1α，HIF-1α)、血管内皮生长因子(Vascular endothelial growth factor，VEGF)及促红细胞生成素(Erythropoietin，EPO)扮演着十分重要的角色。目前，关于HIF-1α、VEGF及EPO的研究主要集中在急慢性高原病及肿瘤等方面，而对于移居高原不同时间下健康习服和适应人群HIF-1α、VEGF及EPO表达变化规律的研究角度不一。本研究通过检测移居高原不同时间下健康汉族HIF-1α、VEGF及EPO的mRNA及蛋白表达水平，并与平原健康汉族做对照，初步明确其表达意义。

1材料与方法

1.1研究对象

选取移居青海省果洛藏族自治州(平均海拔4，200m)健康汉族120例及平原(西安地区，平均海拔400m)健康汉族30例。分为五组：移居高原1周组、1月组、 1年组、5年组及平原组，每组各30例。以上各组人群年龄、性别比例无统计学差异，无吸烟、饮酒史，无心、肺、脑、肝、肾等基础病史，经常规体检(行血常规、肝功、肾功、胸片、心电图、肺功能等检查)证实身体健康，所有实验参与者均知情同意并签字。……