熟眠胶囊对失眠模型大鼠脑内不同组织5-HT1a、5-HT2a受体表达的影响

王冰梅1，许峰2，王中男2*

(1.长春中医药大学基础医学院，长春 130117；2.长春中医药大学第一附属医院，长春 130117)

摘要：目的观察熟眠胶囊对失眠大鼠脑内5-HT1a、5-HT2a受体表达的影响。方法将成年雄性Wistar大鼠60只随机分为正常对照组(N组)、失眠模型组(M组)、地西泮组(D组)、熟眠胶囊高、中、低组(SM-H、SM-M、SM-L组),每组10只，采用腹腔注射PCPA法造模。造模成功后，给药组分别给予熟眠胶囊(28，14，7 g/kg)、地西泮(0.525 mg/kg)，N组、M组给予相应容量的蒸馏水，连续给药7 d,分别观察各组大鼠下丘脑、海马内5-HT1a、5-HT2a受体mRNA表达量的变化。结果与N组相比，M组下丘脑、海马5-HT1a表达量明显降低、5-HT2a表达量明显升高，差异有统计学意义(P<0.05、P<0.05);与M组相比，D组下丘脑、海马5-HT1a表达量明显升高、5-HT2a表达量明显降低,差异有统计学意义(P<0.05、P<0.05)；与M组相比，SM-H、SM-M组下丘脑、海马5-HT1a表达量明显升高(P<0.05、P<0.05)、5-HT2a表达量明显降低(P<0.05、P<0.05),差异有统计学意义。结论熟眠胶囊可以通过调节失眠模型大鼠下丘脑、海马中5-HT1a、5-HT2amRNA的表达发挥治疗作用。

关键词：熟眠胶囊；失眠；5-羟色胺1a受体；5-羟色胺2a受体

DOI:10.13463/j.cnki.jlzyy.2015.03.022

中图分类号：R256.23文献标志码： A

文章编号：1003-5699(2015)03-0286-03

基金项目:吉林省科学技术厅课题(20110930)。

作者简介:王冰梅(1970-)，女，博士，副教授，主要从事中西医结合基础研究。

收稿日期:(责任编辑：张瑞彬2014-02-10)

*通信作者:王中男，电话-(0431)86172503，电子信箱-zhongnanw@sina.com

Effects of Shu-Mian Capsule on 5-HT1a、5-HT2areceptor mRNA expression of

different brain tissue of insomnia rat

WANG Binmei1,XU Feng2,WANG Zhongnan2*

(1.Department of Physiology,College of Basic Medical,Changchun University of TCM,Changchun 130117,China;

2.First Affiliated Hospital of Changchun University of TCM,Changchun 130117,China)

Abstract:ObjectiveTo explore the effect of Shu-Mian Capsule on the expression of 5-HT1a and 5-HT2a receptors in Insomnia rats’ brain.MethodsSixty male Wistar rats were randomly divided into control (N group),insomnia model (M group),Diazepam (D group),and high,middle and low dose group of Shu-Mian Capsule groups(SM-H、SM-M、SM-L group respectively),with 10 cases in each,and the insomnia model rats were made by intraperitoneal injection of PCPA.The rats of SM-H、SM-M、SM-L group were subsequently administrated with Shu-Mian Capsule at a dose of 28 g/kg,14 g/kg,7 g/kg respectively,while rats in D group with Diazepam at a dose of 0.525 mg/kg,and rats in M and N groups with the same volume of distilled water.The treatment lasted for 7 days.Then the expression of 5-HT1a and 5-HT2a receptors mRNA in hypothalamus and hippocampus in each rat were investigated.ResultsCompared with the control (N) group,the expression of 5-HT1a mRNA in hypothalamus and hippocampus in rats from M group decreased significantly,while the 5-HT2a mRNA increased significantly(P<0.05,P<0.05).Compared with M group,the expression of 5-HT1a mRNA in hypothalamus and hippocampus in rats from D group increased significantly,while the 5-HT2a mRNA decreased significantly (P<0.05,P<0.05).Compared with M group,the expression of 5-HT1a mRNA in hypothalamus and hippocampus in rats from SM-H and SM-M groups increased significantly,while the 5-HT2a mRNA decreased significantly (P<0.05,P<0.05).ConclusionThe therapeutic effect of Shu-Mian capsule maybe performed by the regulating of the expression of 5-HT1a and 5-HT2a mRNA in the insomnia model rats’ hypothalamus and hippocampus.

Keywords:Shu-Mian Capsule;Insomnia;serotonin 1a receptor;serotonin 2a receptor

失眠可引起人或动物出现疲劳、思维紊乱，常伴不良的情绪变化。5-羟色胺(5-HT)是睡眠调节最为重要的神经递质之一，脑组织内5-HT1a、5-HT2a受体mRNA的表达量可以反映5-HT对睡眠的调节机制。熟眠胶囊在临床上治疗失眠症疗效确切。本实验通过研究熟眠胶囊对失眠模型大鼠下丘脑、海马内5-HT1a、5-HT2a受体mRNA的表达量的影响，探讨其改善睡眠的作用机制。

1实验材料

1.1实验动物成年雄性Wistar大鼠60只，购自吉林大学实验动物中心，合格证号2013A015，体质量180～220 g。动物于实验前1周适应环境，自由进食、饮水。……