解郁开心丹对抑郁模型大鼠脑内单胺类神经递质的影响

周庆莹，刘宏岩*

(长春中医药大学，长春 130117)

摘要：目的观察解郁开心丹对抑郁模型大鼠脑内去甲肾上腺素(NA)、多巴胺(DA)和5-羟色胺(5-HT)含量的影响。方法80只SD大鼠自由饮食，自然光线下适应性喂养1周后，按照 Open-Field评分方法，将得分≤20分、≥130分的大鼠剔除，剩余的大鼠随机分为6组:正常对照组、模型组、阳性药物对照组、中药高剂量组、中药中剂量组、中药低剂量组。后5组通过禁水24 h、禁食24 h、4 ℃冰水游泳5 min、2 h行为限制、夹尾1 min、潮湿环境、昼夜颠倒，7种应激刺激每天随机安排一种，各种刺激不连续出现，连续刺激3周造模。造模后灌胃给药，阳性药物对照组给予盐酸氟西汀混悬液10 mg/d；解郁开心丹高、中、低剂量组分别给予解郁开心丹2.0，1.0，0.5 g/d(给药体积为每100 g体质量给予1.0 mL)。模型组给予等量生理盐水，持续28 d。用酶联免疫法检测大鼠脑内NA，DA，5-HT的含量。结果与模型组比较，中药高剂量组、中药中剂量组大鼠脑内NA，DA，5-HT含量明显增高且P<0.05；中药高剂量组、中药中剂量组与阳性药物对照组比较无明显差异且P>0.05。结论解郁开心丹具有与西药抗抑郁药物氟西汀同样的抗抑郁作用，且抗抑郁作用机制可能与调节大鼠脑内单胺类神经递质的平衡有关。

关键词：解郁开心丹;抑郁模型;单胺类神经递质;去甲肾上腺素;多巴胺;5-羟色胺

DOI:10.13463/j.cnki.jlzyy.2015.03.021

中图分类号：R741文献标志码： A

文章编号：1003-5699(2015)03-0282-04

基金项目:吉林省自然科学基金(201015118)。

作者简介:周庆莹(1985-)，女，硕士，助教，主要从事中医临床基础理论研究。

收稿日期:(责任编辑：张瑞彬2015-02-10)

*通信作者:刘宏岩，电话-18686698557，电子信箱-hong.yancc@163.com

JieYuKaiXinDanwithin the depression model rats on single amine neurotransmitter

ZHOU Qingying,LIU Hongyan*

(Changchun University of Chinese Medicine,Changchun 130117,China)

Abstract:ObjectiveObserve Jie Yu Kai Xin Dan within the depression model rats on norepinephrine (NA),dopamine (DA) and serotonin (5-HT) content.Methods80 SD rats in free diet,natural light,and adaptive feeding for one week.Make the behavioral score by Open-Field scoring method.Exclude the rats who’s score is ≥120 or ≤30,the last rats are randomly divided into six groups.normal control group,model group,Fluoxetine group,Chinese medicine high dose group,Chinese medicine middle dose group,and Chinese medicine low dose group,the last 5 groups was treated by water deprivation for 24 h,fasted for 24 h,4 ℃ ice water swimming 5 min,2 h behavior restrictions,tail clamp 1 min,humid environment,day and night reversed,and the rat was in different stress factors every day which is randomly decided.After 3 weeks’ continuous stimulation,the depression model was made,normal control group were not treated.Intragastric administration was given after the completion of the modeling,positive control group was given fluoxetine hydrochloride suspension 10 mg/d,positive control drug’s high,middle and low dose group were given Jie Yu Kai Xin Dan 2.0 g/d,1.0 g/d,0.5 g/d (Given 1.0 mL dose volume per 100 g body weight).Model control group were given normal saline.for 28 day.Enzyme league immune method to detect the rats NA,DA and 5-HT content.ResultsConclusion Compared with model group,Chinese medicine dose group of rats in high dose group,the traditional Chinese medicine (TCM) in NA,DA and 5-HT content increased obviously and P<0.05;Traditional Chinese medicine (TCM) in high dose group,Chinese medicine dose group compared with positive drug control group no significant difference(P>0.05).ConclusionJie Yu Kai Xin Dan with western medicine antidepressants fluoxetine same antidepressant effect,and the antidepressant mechanism may be related to regulating single amine neurotransmitter balance in rats.

Keywords:Jie Yu Kai Xin Dan;Depression model;Single amine neurotransmitter;NA;DA;5-HT

抑郁症是现代医学病……