培育颗粒对人绒毛滋养细胞侵袭与增殖的作用

王婧瑶1，孙丽萍1*，赵丕文1，梁欣韫2*

(1.北京中医药大学人体机能系，北京 100029；2.首都医科大学附属北京妇产医院中医科，北京 100026)

摘要：目的观察培育颗粒对人绒毛滋养细胞的侵袭和增殖功能的影响。方法取人妊娠5～10周正常胎盘绒毛,进行滋养细胞培养。正常23～25 d雌性SD大鼠药物灌胃，取药理血清。培养液加20%血清，分组培养48 h后检测指标。用Transwell侵袭实验检测培育颗粒对滋养细胞侵袭功能的影响，MTT法检测培育颗粒对滋养细胞增殖能力的影响,流式细胞术检测培育颗粒对滋养细胞增殖核抗原(PCNA)表达的影响。结果Transwell侵袭实验检测结果表明,培育颗粒血清各剂量组与正常组侵袭细胞数均有差异,中剂量组侵袭细胞数最多。MTT法检测结果表明，培育颗粒血清各剂量组与正常组吸光度均有差异,中剂量组吸光度最高。流式细胞术检测结果表明，培育颗粒血清各剂量组与正常组PCNA阳性率均有差异，中剂量组PCNA阳性率最高。结论培育颗粒可促进人绒毛滋养细胞侵袭与增殖功能，可能与负反馈调节有关。

关键词：培育颗粒；侵袭；增殖；滋养细胞；负反馈调节

DOI::10.13463/j.cnki.jlzyy.2015.07.022

中图分类号：R714.21文献标志码： A

文章编号：1003-5699(2015)07-0719-04

基金项目:国家自然科学基金青年

作者简介:王婧瑶(1987-)，女，硕士研究生，主要从事中医药与生殖研究。

*通信作者:孙丽萍，电话-13681179069,电子信箱-slp201070@163.com

梁欣韫，电话-(010)52276423,电子信箱-fredalxy@163.com

Influence of the Peiyu particles on invasion and proliferation of human trophoblast cell

WANG Jingyao1,SUN Liping1*,ZHAO Piwen1,LIANG Xinyun2*

(1.Function of Human Body Department Beijing University of Chinese Medicine,Beijing 100029,China;

2.TCM Department Beijing Obstetrics and Gynecology Hospital of Capital Medical University,Beijing 100026,China)

Abstract:ObjectiveTo observe the Effect of the Peiyu particles on invasion and proliferation capabilities of human trophoblast cell.MethodsPlacental villi from normal pregnancy of 5 to 10 weeks were taken to culture trophoblast cells.Twenty percent pharmacological serum obtained from Peiyu particles-administered normal female SD rats (aged 23 to 25 days) was added in nutrient solution for culture.After being divided into groups,trophoblast cells were cultured for 48 hours.Then,invasion cell number,absorbance and PCNA expression rate of trophoblast cell in all groups were analyzed by TW invasion assay,MTT assay,and flow cytometry,respectively.ResultsTW assay results show that invasion cell number of each dose group of Peiyu particles is different from that of the normal group,and the invasion cell number of medium dose group is the highest.MTT assay results show that each dose group of Peiyu particles is different from the normal group in absorbance with absorbance of medium dose group being the highest.Flow cytometry results also show that there are differences between each dose group of Peiyu particles and the normal group in PCNA expression rate,and the PCNA expression rate of medium dose group is the highest.ConclusionPeiyu particles can enhance invasion and proliferation abilities of human trophoblast cell,and its effect may be dose-dependent.

Keywords:Peiyu particles;invasion;proliferation；trophoblast cell;negative feedback to adjust

中医认为早孕期间自然流产主要由于肾气不足，胎失所系，脾气虚弱，胎失所养，临床辨证以肾虚和脾肾两虚多见。……