南瓜多糖对D-半乳糖致脑老化模型小鼠SOD、CAT、GSH-PX、MDA的影响

高鹏，颜国华

(郑州澍青医学高等专科学校,郑州 450064)

摘要：目的探讨南瓜多糖对D-半乳糖致脑老化模型小鼠SOD、CAT、GSH-PX、MDA的影响。方法将小鼠随机分为6组,分别为空白组、模型组、维生素E组，南瓜多糖大、中、小剂量组。空白组每鼠每天颈背部皮下注射生理盐水0.5 mL，其余各组每鼠每天颈背部皮下注射5%D-半乳糖生理盐水液0.5 mL造脑老化模型,连续给D-半乳糖45 d。从第10天开始，分别灌服大、中、小剂量南瓜多糖水溶液、维生素E混悬液和同体积生理盐水,1次/d，连续35 d，于最后1次给D-半乳糖和灌药后2 h，小鼠眼球取血，测定SOD、CAT、GSH-PX、MDA。结果与模型组相比，南瓜多糖大剂量组可显著提高小鼠SOD活力(P<0.01)，南瓜多糖中、小剂量组可明显提高小鼠SOD活力(P<0.05)；南瓜多糖大剂量组可明显提高小鼠CAT活力(P<0.05);南瓜多糖大、中、小剂量组均可显著提高小鼠GSH-PX活力(P<0.01);与模型组相比，南瓜多糖大、中、小剂量组均可显著降低小鼠血浆、肝匀浆中MDA水平(P<0.01);南瓜多糖中、小剂量组均可显著降低小鼠脑匀浆中MDA水平(P<0.01),南瓜多糖大剂量组可明显降低小鼠MDA水平(P<0.05)。结论南瓜多糖能够提高SOD、CAT、GSH-PX活力，降低MDA水平，具有一定的抗氧化作用。

关键词：南瓜多糖；D-半乳糖；脑老化；小鼠

DOI::10.13463/j.cnki.jlzyy.2015.07.020

中图分类号：R339.3文献标志码： A

文章编号：1003-5699(2015)07-0713-03

基金项目:郑州澍青医学高等专科学校重点课题(12002)。

作者简介:高鹏(1982-)，男，大学本科，讲师，主要从事基础中医学的教学与研究工作。

收稿日期:(责任编辑：张瑞彬2014-10-25)

Effect of Pumpkin polysaccharide on SOD,CAT,GSH-PX and MDA in

aging mice model induced by D-galactose

GAO Peng,YAN Guohua

(Zhengzhou Shuqing Medical College,Zhengzhou 450064,China)

Abstract:ObjectiveTo observe the effect of Pumpkin polysaccharide on SOD，CAT，GSH-PX and MDA in aging mice model induced by D-galactose.MethodMice were randomly divided into 6 groups,respectively for the blank group,model group,vitamin E,pumpkin polysaccharide large,medium and small dose group.Blank group each rat cervical back subcutaneous injection of saline every day 0.5ml,groups each rat carotid every day back subcutaneous injection of 5% D-galactose saline solution 0.5 mL brain aging model.Straight to the D-galactose 45 days.Starting from the 10 th day,filling and respectively take large,medium and small dose of pumpkin polysaccharide aqueous solution mixed suspension,vitamin E and with normal saline,volume 1 times a day,35 days in a row,in the last one to D-galactose and 2 h after feeding,eyeball blood in mice,the determination of SOD,CAT and GSH-PX and MDA.ResultsCompared with model group,the pumpkin polysaccharide large dose group can significantly increase the SOD activity in mice (P<0.01),the pumpkin polysaccharide,small dose group can obviously increase the SOD activity in mice (P<0.05);Pumpkin polysaccharide large dose group can obviously improve the CAT activity in mice (P<0.05);Large,medium and small dose group of the pumpkin polysaccharide can significantly increase gsh-px activity in mice (P<0.01);Compared with model group,large,medium and small dose group of the pumpkin polysaccharide can significantly decrease the MDA level in plasma,liver homogenate in mice (P<0.01);Pumpkin polysaccharide in medium and small dose group can significantly reduce the little mouse brain homogenate MDA level (P<0.01),pumpkin polysaccharide large dose group can obviously reduce the level of MDA in mice (P<0.05).ConclusionPumpkin polysaccharide could improve vitality of SOD,CAT,GSH-PX,and reduce the level of MDA,it had a certain antioxidant.

Keywords:Pumpkin polysaccharide;D-galactose;laneuria;rat

南瓜是葫芦科南瓜属植物南瓜的果实，营养全面，临床具有多种药用价值[1]。南瓜多糖是南瓜有效成分中重要的活性物质，近年来研究证实[2]，南瓜多糖在降糖、降血脂、抗肿瘤及免疫调节等方面具有明显的药理作用,本研究主要从南瓜多糖对D-半乳糖致脑老化模型小鼠超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)、丙二醛(MDA)的影响方面进行研究，旨在探讨南瓜多糖对衰老小鼠自由基代谢的影响。

1材料与方法

1.1药品及试剂南瓜多糖，西安天园生物制剂厂生产，批号20100624;维生素E，郑州龙生化工产品有限公司生产，批号20111042；……