黑逍遥散联合肾四味对乳腺癌大鼠类更年期症状的影响

付烊1,运强2,朱学明1*

(1.襄阳市中医院肿瘤科，湖北 襄阳 441000;2.湖北中医药大学附属襄阳医院检验科，湖北 襄阳 441000)

摘要：目的观察黑逍遥散联合肾四味预防他莫昔芬引起的乳腺癌大鼠的类更年期症状的影响。方法60只大鼠腹腔注射MNU溶液,构建大鼠的乳腺癌模型。大鼠随机分为3组：A组(空白对照组)20只，B组(他莫西芬对照组)20只，C组(黑逍遥散联合肾四味组)20只。比较3组大鼠的生存率、行为学评分、体质量、血脂和激素水平的变化。结果经过24周,A组大鼠生存率35.00%(7/20),B组和C组大鼠生存率均为75.00%(15/20)。3组大鼠的生存率有统计学意义(P<0.05)。A组和C组大鼠的行为学评分和雌激素、孕激素水平无统计学意义(P>0.05)，与A、C组大鼠相比，B组大鼠的行为学评分和雌激素、孕激素明显下降(P<0.05)。A组和B组大鼠的体质量无统计学意义(P>0.05),C组大鼠的体质量明显高于A、B组大鼠(P<0.05)。各组大鼠的CHO、TG、HDL-C和LDL-C水平无统计学意义(P>0.05)。结论黑逍遥散联合肾四味能有效预防乳腺癌大鼠因应用他莫昔芬导致的类更年期症状，且安全性较高。

关键词：乳腺癌;黑逍遥散;肾四味;他莫昔芬;类更年期症状

DOI::10.13463/j.cnki.jlzyy.2015.07.021

中图分类号：R737.9文献标志码： A

文章编号：1003-5699(2015)07-0716-03

基金项目:襄阳市科技局指导性项目(2014襄科业)。

作者简介:付烊(1979-)，女，主治医师，主要从事中医药防治肿瘤研究。

收稿日期:(责任编辑：张瑞彬2014-11-13)

*通信作者:朱学明，电子信箱-fuyang197901@163.com

<1)，且各件产品是否为不合格品相互独立．

Effect of Heixiaoyaosan combined with Shensiwei on climacteric syndrome of rats with breast cancer

FU Yang1,YUN Qiang2,ZHU Xueming1*

(1.Oncology Department,Xiangyang Hospital of TCM,Xiangyang 441000,Hubei Province,China;

2.Clinical Laboratory,Xiangyang Hospital Affiliated to Hubei University of Chinese Medicine,

Xiangyang 441000,Hubei Province,China)

Abstract:ObjectiveTo study the preventive effect of heixiaoyaosan combined with shensiwei on the climacteric syndrome of rats induced by tamoxifen during the treatment of breast cancer.Methods60 S-D rats were given MNU through ntraperitoneal injection to build breast cancer models,all rats were dividened into 3 groups randomly,20 in group A (control group),20 in group B (Tamoxifen group) and 20 in group C (Heixiaoyaosan combined with Shensiwei group).Compare the survival rate,behavioral grade,body mass,blood fat and concentration of estrogen and progestin among 3 groups.ResultsAfter 24 w,the survial rate of group A is 35.00%(7/20),of group B and C are both 75.00%(15/20),the difference among 3 groups has statistical significance(P<0.05).Group A and group C have no statistical difference in behavioral grade as well as blood fat and concentration of estrogen and progestin(P>0.05),those in group B lower,the difference has statistical significance(P<0.05).Group A and group B have no statistical difference in body mass(P>0.05),it is higher in group B,the difference has statistical significance(P<0.05).The difference of CHO,TG,HDL-C and LDL-C among three groups has no statistical significance(P>0.05).ConclusionHeixiaoyaosan combined with Shensiwei can prevent the climacteric syndrome of rats induced by tamoxifen,and security is reliable.

Keywords:breast cancer;Heixiaoyaosan;Shensiwei;Tamoxifen(TMX);climacteric syndrome

现代医学研究表明乳腺癌的发生发展与内分泌有关，采用雌激素受体拮抗剂或芳香化酶抑制剂成为治疗乳腺癌的重要手段[1-2]。目前常用雌激素拮抗剂来阻断与其受体的结合，代表药是他莫昔芬(TMX)[3]，他莫昔芬抗雌激素和弱雌激素作用会导致患者体内激素水平紊乱，患者体内雌激素水平降低，出现一系列类更年期症状，严重影响患者的生活质量，不利于治疗的顺利进行[4-5]。……