田建平 胡远艳 张吉贞 张名楠 陈国良

摘要

[目的]對海南和广东2省6个产地的华南忍冬分子地理标识及生态因子的关系进行研究，以寻找其内在联系。[方法] 应用主成分分析和数量分类技术对华南忍冬6个产地的温度、水分、光照、经纬度等地理生态因子进行分析，通过psbAtrnH序列测序和分子指纹图谱分析，寻找不同产地的差异。[结果]可以将6个产区分为3部分，其中屯昌和琼中产区以年均降水量贡献值最大，韶关产区则以7月均最高温、经纬度贡献值最大;海口、文昌和湛江则以年均气温、年均相对湿度、1月气温等贡献值最大。基于psbAtrnH 序列对不同产地华南忍冬进行分子指纹图谱鉴别及分析，结果表明，利用 psbAtrnH 序列比对分析发现，华南忍冬不同产地间序列位点存在较大差异，保守位点有267 bp，占序列总长度的60.2%; 变异位点有182 bp，占序列总长度的39.8%， NJ tree 分析表明，华南忍冬2省6产区可分为3支，与基于地理生态因子的分类具有很强的相关性。[结论]该研究为进一步研究山银花的生态型和品质变异的生物学本质提供科学依据。

关键词 华南忍冬;生态因子;psbAtrnH序列;指纹图谱

中图分类号 S188  文献标识码

A  文章编号 0517-6611（2015）03-038-03

Study on the Correlation between Gene Sequence Characteristics of psbAtrnH and Ecological Factors of  Lonicera confusa

TIAN Jianping¹，HU Yuanyan²，ZHANG Jizhen²  et al

（1.Department of paharmacy，Hainan medical college，Haikou，Hainan 571199 ;2.Key Laboratory for Tropical Biological Resources，MOE，Hainan University，Haikou，Hainan 570228;3.Materials and Chemical Engineering，Hainan University，Haikou，Hainan 570228）

Abstract   [Objective]To discuss the relationship between molecular geographical indications and ecological factors of L.confusa，  the  relative datas from the six regions of Hainan and Guangdong province were studied.[Method]By using of principal component analysis（PCA） and quantitative classification technology，  geographical and ecological factors （ including temperature，moisture，illumination，latitude and longitude，etc） from  six areas  of L.confusa was analyzed. Through psbAtrnH sequencing and molecular fingerprintiny analysis， the difference from different habitats was found.[Result] The results showed that the six regions could be divided into three parts，and the Tunchang and Qiongzhong County have the maximum contribution value with the average annual rainfall ，Shaoguan region have the maximum contribution value with  the most high temperature in July，latitude and longitude. Haikou，Wenchang and Zhanjiang have the maximum contribution value with the annual temperature，relative humidity，air temperature of January，etc.Molecular fingerprint identification and analysis of L.confusa  based on the psbAtrnH sequences had been researched in different areas，the results had been showed that，there had existed sequence differences between different habitats， and the conservative sites were 267 bp，the mutation locus were 182 bp; NJ tree analysis showed that six areas of  L.confusa in southern China can be divided into three branches too，and it had  the strong correlation  between the results of classification based on geographical and ecological factors.[Conclusion] The study    provided the scientific basis for the biological nature for the further studies of variation of ecotypes and quality of Lonicerae flos.

Key words  Lonicera confuse; Ecological factors; psbAtrnH; Fingerprint

基金项目 海南省教育厅科学基金资助项目（Hjkj2012-29）。

作者简介 田建平（1972- ），男，湖南常德人，副教授，从事中药资源学研究。

收稿日期 20141204

道地藥材品质特征及其成因的系统研究一直是中药材科学研究中的热点和难点之一^[1]。由于遗传和生态因子等因素的共同作用导致种内变异是中药材品质不同的主要原因^[2]。……