于海宁，陶艳红，冯莹超，华允芬，沈生荣，单伟光

(1.浙江工业大学 药学院，浙江 杭州 310014； 2.浙江大学 生物系统工程与食品科学学院，浙江 杭州 310058)

蛋白酶结合自溶酶提取南极磷虾油工艺研究

于海宁1，陶艳红1，冯莹超1，华允芬1，沈生荣2，单伟光1

(1.浙江工业大学 药学院，浙江 杭州 310014； 2.浙江大学 生物系统工程与食品科学学院，浙江 杭州 310058)

摘要：南极磷虾油含有丰富的磷脂型ω-3不饱和脂肪酸，具有极大的开发价值.南极磷虾含有丰富的自溶酶体系，其自溶作用是否有利于活性物质的提取尚不清楚.采用木瓜蛋白酶对南极磷虾油进行提取，探索自溶酶体系在南极磷虾油提取过程中的作用.结果表明：南极磷虾自溶作用有利于南极磷虾油的提取，木瓜蛋白酶结合自溶酶体系共同作用可取得最佳提取效果.以EPA与DHA质量分数之和为检测指标，探讨了不同酶解温度、料液比、加酶质量分数、酶解时间和pH对南极磷虾油提取效果的影响.先通过单因素实验，再通过正交实验，分析确定了最佳提取工艺条件：酶解温度50 ℃，料液比1∶22 g/mL，加酶质量分数1.25%，酶解时间2 h，pH7.0.在此条件下提取的虾油，EPA和DHA质量分数之和为75.77%。

关键词：南极磷虾；木瓜蛋白酶；自溶；提取

Study on extraction of Antarctic krill oil by protease combined with autolysis

YU Haining1, TAO Yanhong1, FENG Yingchao1, HUA Yunfen1,

SHEN Shengrong2, SHAN Weiguang1

(1.College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China；

2.School of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China)

Abstract:Antarctic krill oil is rich in phospholipid type of ω-3 unsaturated fatty acids, and has a great value of development. There are many kinds of self-plasmin in antarctic krill, till now, whether the autolysis of Antarctic krill accelerated extracting of Antarctic krill oil is unclear. In this work, papain was used to extract Antarctic krill oil, while the role of self-lysosomal system in the Antarctic krill oil extraction process was evaluated. The results showed that krill autolysis was beneficial for Antarctic krill oil extraction, and lysosomal system combined with papain can work together to achieve the best extraction results. The content of eicosapentaenoic acid(EPA) and docosahexaenoic acid(DHA) in shrimp oil was investigated with respect to extraction parameters such as enzymatic hydrolysis temperature, solid-liquid ratio, enzyme dosage, hydrolysis time and pH. One-factor-at-a-time experiments followed by orthogonal test analysis were carried out to achieve the optimal extraction conditions as below: At a temperature of 50 ℃, solid-liquid ratio of 1∶22 g/mL, enzyme dosage of 1.25%, pH7.0 hydrolysis 2 hours. Under the optimal conditions, the total content of EPA and DHA in the shrimp oil is up to 75.77%。

Keywords:Antarctic krill; papain; autolyzed; extraction

南极磷虾渔业始于20世纪60年代，80年代达到鼎盛期，1982年上岸量达到历史最高的52.8 万吨.据估计，南极磷虾的生物量为6.5~10.5 亿吨[1-2]，是整个南大洋生态系统能量和物质流动的关键环节[3-4]，具有巨大的开发和利用潜力.南极磷虾营养比例适中，必需氨基酸占总氨……