刘亚莉等

[摘要] 目的 研究高脂饮食喂养小鼠内脏脂肪和皮下脂肪组织中PAI-1、FOXC2及FOXO1表达水平，探讨不同类型肥胖的机制。 方法 20只小鼠随机分为对照组和高脂组，分别给予正常饮食和高脂饮食喂养12周。测定血清PAI-1以及附睾周围组织及皮下脂肪中PAI-1、FOXC2及FOXO1 mRNA的表达水平。 结果 高脂组小鼠体质量、血清PAI-1水平均显著高于对照组。组内比较小鼠附睾周围脂肪组织PAI-1、FOXC2mRNA表达显著高于皮下脂肪组织；FOXO1mRNA表达显著低于于皮下脂肪组织，差异有统计学意义。 结论 正常体重小鼠和肥胖小鼠血清PAI-1水平表达不同，内脏脂肪和皮下脂肪PAI-1、FOXC2及FOXO1表达也存在差别，这种差别可能是不同类型肥胖的机制之一。

[关键词] 血浆纤溶酶原激活物抑制物-1；FOXC2；FOXO1

[中图分类号] R589.2 [文献标识码] A [文章编号] 2095-0616（2014）16-26-04

Study of expression of PAI-1， FOXC2 and FOXO1 in adipose tissue of mice with high fat diet

LIU Yali1 YI Jiali2 LIU Jianhui3 NIU Lifeng3

1.Health Inspection Faculty Working Office of Liaoning Medicine Career Academy， Shenyang 110101，China；2.Dean's Office of Liaoning Medicine Career Academy， Shenyang 110101，China；3.Department of Inspection Technology， Liaoning Medicine Career Academy， Shenyang 110101，China

[Abstract] Objective To study the expression level of PAI-1， FOXC2 and FOXO1 in high fat diet of visceral fat and subcutaneous fat tissue，and to investigate the mechanism of different types of obesity. Methods 20 mouse were randomLy divided into control group and high fat group， and given normal diet and high fat diet for 12 weeks. Serum PAI-1，the expression level of PAI-1， FOXC2 and FOXO1 mRNA in epididymal tissue and subcutaneous fat were determined. Results The body weight， serum PAI-1 levels of high fat group were significantly higher than those of the control group. Within the group， PAI-1，FOXC2， mRNA expression of mouse epididymal adipose tissue were significantly higher than those of the subcutaneous adipose tissue， and the expression of FOXO1mRNA of mouse epididymal adipose tissue was significantly lower than that of subcutaneous adipose tissue， with statistical significance difference. Conclusion Expression levels of serum PAI-1 are different between normal weight mice and obese mice，and expression of fat PAI-1， FOXC2and FOXO1 in visceral fat and subcutaneous are also different.This difference may be one of the mechanisms of different types of obesity.

[Key words] Plasminogen activator inhibitor -1； FOXC2； FOXO1

随着生活方式和饮食习惯的改变，肥胖已成为全球的常见病，而与肥胖相关的冠心病、高血压、2型糖尿病等许多代谢性疾病则成为严重危害人类健康的杀手。研究发现[1]肥胖者脂肪堆积部位的差异强烈影响上述疾病的发病风险，与外周肥胖相比，腹型肥胖（内脏肥胖，即内脏脂肪过度堆积）更易造成胰岛素抵抗、糖脂代谢紊乱和心血管疾病。因此，关于不同部位脂肪组织差异性的研究对于揭示相关疾病的发病机制具有重要作用。血浆纤溶酶原激活物抑制物-1（plasminogen activator inhibitor，PAI-1）是一种由脂肪细胞合成和分泌的细胞因子[2]，PAI-1水平的升高将直接导致纤溶活性受损，与肥胖、胰岛素抵抗、2型糖尿病及心血管疾病密切相关[3]。近来发现的翼状螺旋叉头转录因子家族的叉头框（Forkhead box）蛋白C2（FOXC2）和叉头框蛋白O1（FOXO1）在调控胰岛素诱导的PAI-1表达方面发挥重要作用，但具体的调控机制尚不明确。……