关键词金顶侧耳;磷酸葡萄糖变位酶；克隆;生物信息学

中图分类号Q785 文献标识码A 文章编号 1007-7731（2025）15-0108-06

DOI号10.16377/j.cnki.issn1007-7731.2025.15.026

Cloning and bioinformatics analysis of phosphoglucomutase（PGM） in Pleurotus citrinopileatus

IUI Xiangyun SHI WuqianTANG Hao ZHOU Song LI Yuntao LYU Chaotian LI Huan （School of Food and Bio-engineering，Bengbu University，Bengbu 23303o， China）

AbstractTo explore the characteristics and structure of the phosphoglucomutase （PGM） gene in Pleurotus citrinopileatus，the studywas based on the genomic data of Pleurotus citrinopileatus.The PGM gene wascloned by RT-PCR technology，and the encoded protein was analyzedby bioinformatics，including physicochemical properties， hydrophobicity，secondary and tertiary structures，etc.The results showed that the full length sequence of the PGM （204号 gene in P .citrinopileatus was 1 797 bp，encoding 598 amino acids，with a molecular weight of 66.35 kDa. The isoelectric point of the protein was 5.98，the average hydrophilicity was -0.278 ，and the instability indexwas 29.54， indicatingthat it was anacidic，hydrophilicand stable protein.The protein had no signal peptide，no cleavage site， and no transmembrane helices，belonging toanon-secreted and non-transmembrane protein.There were conserved domains in the amino acid sequence regions，belonging to the PTZoo15O gene family，with 81 phosphorylation sites， located in the cytoplasm.The secondary structure mainly includes α -helices （43.14%） ，irregular curls （35.79% ） extended chains （14.72%） ，and β -folds （6.35% ）; the tertiary structure is mainly composed of alpha helices and irregularcoils，and thestructure isrelatively stable.Theresultsof homologyand phylogenetictree analysis showed that thesimilarityofPcPGMwith Pleurotusostreatus，Pleurotuspulmonarius，Hypsizygusmarmoreusand Lyophyllum shimeji was 78.04% ， 79.7% ， 71.33% and 67.50% ，respectively，and it was most closely related to P . ostreatus and P pulmonarius，both belonging to the Auriculariaceae family.There were6proteins that might interact with the PcPGM protein， including hexokinase 1 （HXK1）， glucose-6-phosphate isomerase （GPIC）， α -glucan phosphorylase 1 （ α GP1）， （20 α GP2，UTP-glucose-1-phosphateuridylyltransferase1（UGP1）and UGP2，all of which were related to sugar metabolism.This study provides areference for further exploration of the biological functionandmedicinal value of the PGM gene in P .citrinopileatus.

Keywords Pleurotus citrinopileatus;phosphoglucomutase; cloning;bioinformatic

金顶侧耳（Pleurotuscitrinopileatus）是一种珍贵的食、药用真菌，主要分布于云南、广西、四川等地1。其凭借高蛋白、低糖、低脂的营养特性，在膳食结构中占据重要地位。Zhang等2研究表明，金顶侧耳具有较高的药理活性，其子实体多糖具有免疫增强作用。王沛等3通过液体摇瓶培养技术提取的菌液，有效促进了小鼠体液免疫与细胞免疫功能的提升。随着食用菌全基因组序列的解析，利用现代分子生物学手段调控其多糖合成途径中的关键基因，已成为提升多糖产量的重要策略之一[4-6]。

磷酸葡萄糖变位酶（Phosphoglucomutase，PGM）的核心功能是催化葡萄糖-6-磷酸与葡萄糖-1-磷酸之间的可逆转化。其中，葡萄糖-6-磷酸进入分解代谢过程以产生能量，而葡萄糖-1-磷酸是糖核苷酸的前体，被细胞用于合成各种多糖。PGM在细胞壁和多糖生物合成中具有重要功能7。例如，在灵芝中过表达 α-PGM ，可显著增强PGM及磷酸葡萄糖异构酶基因的转录水平及相关酶活性，从而提高灵芝多糖含量。目前，研究人员已在拟南芥（Arabidopsisthaliana）、玉米（Zeamays）、马铃薯（Solanum tuberosum）[o、广东虫草（Cordycepsguangdongensis）[等植物和真菌中克隆出PGM基因。然而，有关金顶侧耳PGM基因的相关研究较少。为此，本研究以金顶侧耳全基因组数据为基础，通过特异性引物对PGM基因进行克隆与生物信息学分析，为其在食、药用方面的应用提供参考。

1材料与方法

1.1试验材料

金顶侧耳由蚌埠学院食品与生物工程学院提供。取健康菌丝放于离心管中，液氮速冻并于-80° 冰箱保存，用于总RNA提取。……