[摘要]目的：探讨低强度脉冲超声（Low-intensity pulsed ultrasound，LIPUS）联合骨形成蛋白9（Bone morphogenetic protein 9，BMP9）修饰的骨髓间充质干细胞（Bone marrow derived mesenchymal stem cells，BMSCs）对牙槽骨缺损的修复作用。方法：在大鼠上颌第一磨牙近中区造成牙周骨缺损构建牙槽骨缺损大鼠模型，将45只大鼠随机平均分为5组，每组9只，即正常组、对照组、超声治疗组、细胞治疗组、联合治疗组。通过CT扫描评价大鼠牙槽骨再生，通过HE染色、Masson染色和免疫组织化学染色观察大鼠骨缺损修复程度。利用生物发光成像在体内追踪BMSCs。结果：微型CT结果显示，与对照组相比，超声治疗组，细胞治疗组，联合治疗组促进了更多的新骨形成、BV、Tb.N增加、Tb.Sp降低，其中联合治疗组的新骨形成率明显增加（P＜0.05）；HE染色和Masson染色结果显示，在三组治疗组中，均可以观察到结缔组织、新血管形成和钙沉积物，联合治疗组中更明显；免疫组化染色结果显示，在三组治疗组中，联合治疗组COL-I 和骨桥蛋白表达增高更明显。生物发光显像显示LIPUS联合BMP9修饰的BMSCs组的荧光信号明显多于其他组，有较多的BMSCs定植于牙槽骨缺损区。结论：LIPUS联合BMP9修饰的BMSCs可以促进牙槽骨再生，对牙槽骨缺损修复的效果更好。

[关键词]低强度脉冲超声（LIPUS）；骨形成蛋白9（BMP9）；骨髓间充质干细胞（BMSCs）；牙槽骨修复；联合作用

[中图分类号]R781.42" " [文献标志码]A" " [文章编号]1008-6455（2025）06-0006-05

Study on the Repair Effect of LIPUS Combined with BMP9 Modified BMSCs on Alveolar Bone Defects in Rats

LIN Lirong， LI Zhenxing

（ Shijiazhuang Medical College， Shijiazhuang 050000， Hebei， China ）

Abstract： Objective" Exploring the repair effect of LIPUS combined withBone morphogenetic protein 9（BMP9）modified Bone marrow derived mesenchymal stem cells（BMSCs）on alveolar bone defects. Methods" A rat model of alveolar bone defect was constructed by creating periodontal bone defects in the mesial area of the first maxillary molar in rats. 45 rats were randomly divided into 5 groups， with 9 rats in each group， namely the normal group：normal group， control group， beyond treatment group， cell therapy group， combination therapy group. Eight weeks after surgery， the rats were euthanized and samples were collected. Evaluate alveolar bone regeneration in rats using CT， and the degree of bone defect repair was observed using HE staining， Masson staining， and immunohistochemical staining. BMSCs were tracked in vivo using bioluminescence imaging. Results" The micro CT results showed that compared with the control group， the treatment group， cell therapy group， and combination therapy group promoted more new bone formation， BV， and Tb N increases， Tb Sp decreased， with a significant increase in new bone formation rate in the combination treatment group （P＜0.05）. The results of HE staining and Masson staining showed that in all three treatment groups， connective tissue， neovascularization， and calcium deposition were observed， with a more pronounced effect in the combination treatment group. The immunohistochemical staining results showed that among the three treatment groups， the combined treatment group had a more significant increase in COL-I and osteopontin expression. Bioluminescence imaging showed that the fluorescence signal of the LIPUS combined with BMP9 modified BMSCs group was significantly higher than that of the other groups， and more BMSCs were implanted in the alveolar bone defect area. Conclusion" Lipus combined with BMP9 modified BMSCs can promote alveolar bone regeneration and have a better effect on repairing alveolar bone defects.

Key words： low-intensity pulsed ultrasound（ LIPUS ）; bone morphogenetic protein 9 （ BMP9 ）; bone marrow derived mesenchymal stem cells（ bmscs ）; alveolar bone repair; combined effect

牙周炎是人类最常见的口腔疾病之一，牙槽骨再生是牙周炎治疗中的关键问题[1]。骨髓间充质干细胞（BMSCs）是维持骨代谢的主要细胞。BMSCs表现出多系分化能力，包括软骨细胞和成骨细胞，其可通过迁移到骨损伤部位对骨缺损进行修复[2]。……