高国龙 张兴旺 姜子健 黄家风 都业娟

摘要 為了明确木尔坦棉花曲叶病毒cotton leaf curl Multan virus （CLCuMuV）在新疆的发生分布，对采自新疆13个地（州）的1 166份蜀葵、苘麻、棉花疑似病株进行CLCuMuV分子鉴定和带毒检测，并对3种寄主上同期采集的1 588头烟粉虱进行隐种鉴定和带毒检测。结果显示，CLCuMuV DNA-A组分及其伴随的DNAβ卫星分子均可侵染蜀葵、苘麻、棉花;基于DNA-A及DNAβ组分的进化分析表明，新疆3种寄主上发生的CLCuMuV与国内已报道的CLCuMuV 进化关系一致;蜀葵、苘麻、棉花上CLCuMuV带毒率存在差异，分别为27.87%、33.33%和14.95%;MEAM1和MED隐种为新疆烟粉虱主要种群，分别占26.70%和66.44%，带毒率分别为21.46%和17.63%，说明MED隐种为新疆烟粉虱优势种群，2个隐种均可携带CLCuMuV且带毒率相近;MEAM1隐种在蜀葵、苘麻、棉花上带毒率分别为21.39%、0和28.21%，MED隐种分别为19.61%，8.47%和11.18%，表明2个隐种在不同寄主上的带毒率存在差异。本研究明确了新疆地区侵染蜀葵、苘麻和棉花的CLCuMuV发生及分布情况，并明确新疆烟粉虱主要种群MEAM1和MED 隐种在3种寄主上的分布及带毒情况，为新疆棉区防控CLCuMuV发生严重危害提供科学依据。

关键词 CLCuMuV;棉花;蜀葵;苘麻;烟粉虱

中图分类号： S435.61

文献标识码： A

DOI： 10.16688/j.zwbh.2021154

Abstract In order to determine the distribution of cotton leaf curl Multan virus （CLCuMuV） in Xinjiang， 1 166 suspected plants of Alcea rosea， Abutilon theophrasti and cotton collected from 13 prefectures （autonomous prefectures） in Xinjiang were investigated by molecular identification and CLCuMuV detection， and the total of 1 588 Bemisia tabaci were also collected from the three hosts to determine the cryptic species of B.tabaci and their viruliferous rate of CLCuMuV by PCR. The results showed that A.rosea， A.theophrasti and cotton could be infected by CLCuMuV DNA-A and its beta satellite DNAβ. CLCuMuV DNA-A and its DNAβ from the three hosts in Xinjiang were closely related to the CLCuMuV isolates from other provinces in China based on phylogenetic tree analysis. The rate of CLCuMuV infection in A.rosea， A.theophrasti and cotton was 27.87%， 33.33% and 14.95%， respectively. The population of B.tabaci in Xinjiang was mainly comprised of MEAM1 and MED cryptic species. MEAM1 accounted for 26.70% with 21.46% viruliferous rate， and MED accounted for 66.44% with 17.63% viruliferous rate， indicating that MED cryptic species is the dominant population of B.tabaci in Xinjiang. Both MEAM1 and MED could carry CLCuMuV with similar viruliferous rate， the viruliferous rate of MEAM1 in A.rosea， A.theophrasti and cotton was 21.39%， 0 and 28.21%， respectively， and that of MED was 19.61%， 8.47% and 11.18%， respectively. The results showed that the viruliferous rates of MEAM1 and MED on different hosts were different. In conclusion， the occurrence and distribution of CLCuMuV in A.rosea， A.theophrasti and cotton in Xinjiang were determined， and the distribution and viruliferous rates of MEAM1 and MED on the three hosts were also determined， which provides scientific basis for the prevention and control of CLCuMuV in Xinjiang.69033D07-FF88-4A2B-9A1F-6EC5CF4241CA

Key words CLCuMuV;cotton;Alcea rosea;Abutilon theophrasti;Bemisia tabaci

棉花曲叶病cotton leaf curl disease（CLCuD）是棉花生长期的毁灭性病害，不仅造成棉纤维长度、细度等品质下降，还严重影响产量，造成的产量损失可达10%～90%[1]。该病害是由烟粉虱Bemisia tabaci（Gennadius）传播的双生病毒科Geminiviridae菜豆金色花叶病毒属Begomovirus的多种病毒引起，通常统称为棉花曲叶病毒cotton leaf curl virus（CLCuV）。CLCuV可侵染其他寄主，并通过烟粉虱传播到棉花上[1]，因此烟粉虱和其他寄主的分布和带毒情况对该病害的传播及流行具有重要作用。……