张倩 成立新 温彩霞 常静 孟瑞霞 李海平 王振 戴桂香

摘要 为了明确巴氏新小绥螨Neoseiulus barkeri溴氰菊酯抗性品系对常用杀虫（螨）剂的交互抗性水平及生物适合度，为巴氏新小绥螨抗药性品系田间应用提供科学依据。本文应用玻片浸渍法测定了巴氏新小绥螨溴氰菊酯抗性品系对12种杀虫（螨）剂的交互抗性，采用种群生命表评价了其生长发育及繁殖情况。结果显示，相对于巴氏新小绥螨敏感品系，溴氰菊酯抗性品系（226.38倍）对供试的其他6种拟除虫菊酯类药剂均存在交互抗性，尤其对氯氟氰菊酯的交互抗性倍数最高，达到2 210.91倍。对噻虫嗪、毒死蜱和乙螨唑的交互抗性分别是116.46、110.71倍和20.43倍，对丁硫克百威、阿维菌素和哒螨灵无交互抗性。对其生物适合度研究发现，溴氰菊酯抗性品系相较于敏感品系，前若螨期和产卵前期显著缩短，产卵期和单雌总产卵量显著增加。上述结果表明，巴氏新小绥螨溴氰菊酯抗性品系对10种常用杀虫（螨）药剂产生了不同水平的交互抗性，且其繁殖率显著增加，推荐在田间推广应用。

关键词 巴氏新小绥螨;溴氰菊酯;抗药性品系;交互抗性;生长发育

中图分类号： S476.2

文献标识码： A

DOI： 10.16688/j.zwbh.2021495

Abstract This study aims to clarify the cross-resistance level of a deltamethrin-resistant strain of the Neoseiulus barkeri to commonly used pesticides and its fitness， and provide scientific basis for the field application of the strain in biological pest control. The cross-resistance of the deltamethrin-resistant strain to 12 commonly used pesticides was measured by slide-dip method， and the growth and development parameters and the fecundity were determined by the life-table analysis. The results showed that the deltamethrin-resistant strain （226.38 fold） of N. barkeri had cross-resistance to all the other six tested pyrethroids， especially the level of cross-resistance to cyhalothrin is the highest which reached 2 210.91 fold. The strain also showed cross-resistance to thiamethoxam， chlorpyrifos and etoxazole， which was 116.46， 110.71 fold and 20.43 fold， respectively， compared to the sensitive strain. There was no cross-resistance to carbosulfan， abamectin and pyridaben. The results of its biological fitness test reveal that the protonymph stage， pre-oviposition stage were significantly shortened， but the oviposition period and the total eggs laid by single female increased in the resistant strain. These results indicated that the deltamethrin-resistant strain of N. barkeri showed different levels of cross-resistance to nine of commonly used pesticides and their fecundity significantly increased， therefore， it could be more widely used as a biological control agent.

Key words Neoseiulus barkeri;deltamethrin;resistant strain;cross-resistance;growth and development

巴氏新小绥螨Neoseiulus barkeri属植绥螨科Phytoseiidae新小绥螨属Neoseiulus，主要分布在温带地区，在澳大利亚、欧洲、以色列等国家和地区被广泛用于防治蓟马和叶螨[1]。该螨对马铃薯腐烂茎线虫Ditylenchus destructor [2]、水稻干尖线虫Aphelenchoides besseyi [3]、西花蓟马Frankliniella occidentalis [4]、柑橘全爪螨Panonychus citri [5]和朱砂叶螨Tetranychus cinnabarinus [6]等有较好的防治效果。因其扩散能力强、死亡率低、发育历期短、易于饲养和大量繁殖等优点，被认为是最有效的生物防治天敌之一[7]。……