裴新国 张帅 张彦超 郑晨 高聪芬 吴顺凡

摘要 为明确我国褐飞虱田间种群对氟啶虫胺腈的抗性现状及生化抗性机制，2017年-2019年采用稻茎浸渍法测定了采集自7省共13个褐飞虱田间种群对氟啶虫胺腈的抗性，并研究了氟啶虫胺腈抗性种群与其他杀虫剂的交互抗性以及增效剂对氟啶虫胺腈的增效效果。结果表明：近3年来褐飞虱对氟啶虫胺腈产生了中等水平抗性（RR=10.3～30.9）。氟啶虫胺腈抗性品系对呋虫胺、噻虫嗪和烯啶虫胺分别产生了9.1倍、7.9倍和4.1倍的低水平交互抗性，与噻嗪酮、毒死蜱、吡蚜酮、三氟苯嘧啶和吡虫啉不存在交互抗性。增效剂PBO对氟啶虫胺腈抗性品系和浙江龙游19（Longyou-19）田间种群分别具有4.2倍和3.8倍的明显增效作用。综上，褐飞虱田间种群已对氟啶虫胺腈产生中等水平抗性。多功能氧化酶参与了褐飞虱对氟啶虫胺腈的代谢抗性。

关键词 褐飞虱;氟啶虫胺腈;抗药性监测;交互抗性;生化抗性机制

中图分类号： S435.112.3

文献标识码： A

DOI： 10.16688/j.zwbh.2021097

Abstract To understand the resistance status and mechanism of field populations of Nilaparvata lugens to sulfoxaflor， we determined the resistance to sulfoxaflor of 13 field populations of N. lugens collected from seven provinces in 2017-2019 by using the rice-stem dipping method. Cross-resistance and synergism tests were also investigated in this study. The results showed that all of the field populations had developed moderate resistance to sulfoxaflor （resistance ratio=10.3-30.9）. Compared with the sensitive strain （SUL-S）， the selected SUL-R strains showed low-level cross-resistance to dinotefuran， thiamethoxam and nitenpyram （9.1-fold， 7.9-fold and 4.1-fold， respectively）. No cross-resistance to buprofezin， chlorpyrifos， pymetrozine， triflumezopyrim and imidacloprid was detected. Bioassays revealed that the P450 inhibitor， piperonyl butoxide （PBO） had 4.2-fold and 3.8-fold synergistic effects on sulfoxaflor to sulfoxaflor-resistant strains and field strains（Longyou-19）. Our results indicated that all of the populations developed moderate resistance to sulfoxaflor. Enhanced metabolism mediated by cytochrome P450 monooxygenases was involved in the resistance of N. lugens to sulfoxaflor.

Key words Nilaparvata lugens;sulfoxaflor;resistance monitoring;cross-resistance;biochemical resistance mechanisms

褐飞虱Nilaparvata lugens （Stl） 属于半翅目飞虱科，是威胁我国水稻安全生产的重要害虫[1]。褐飞虱主要以刺吸和产卵为害水稻，其分泌物覆盖植物表面也会影响水稻的光合作用。此外褐飞虱还可传播水稻病毒病如草状矮缩病和齿叶矮缩病等[2]。目前对褐飞虱的防治主要依赖于化学农药，然而长期、大量、不科学地使用杀虫剂使得褐飞虱田间种群对多种常用杀虫剂产生了不同程度的抗性，导致杀虫剂的田间防治效果显著下降[3]。吡虫啉于20世纪90年代初进入我国市场后，以其优异的防治效果，迅速成为我国防治褐飞虱的主要药剂。……