14种AM真菌对枳生长的影响
2021-12-23张金莲李铭燕康贻豪刘金华陈廷速李贤良宋娟刘升球
张金莲 李铭燕 康贻豪 刘金华 陈廷速 李贤良 宋娟 刘升球






摘 要:在盆栽条件下,以柑橘砧木枳(Fructus aurantii)幼苗为试验材料,在基质中接种从广西土壤中分离得到的不同丛枝菌根(AM)真菌菌株,探讨不同AM真菌对枳根系的侵染能力和植株生长的影响。结果表明:用于试验的14个AM真菌菌株均能与枳根系形成共生关系,平均侵染率为36.16%,其中侵染率最高的菌株是黄雷德克囊霉(Redeckera fulvum, Rf),侵染率高达92.83%,侵染率最低的菌株是副冠球囊霉(Glomus coronatum, Gc),侵染率仅为0.23%;接种黄雷德克囊霉、摩西斗管囊霉(Funneliformis mosseae, Fm2)和副冠球囊霉这3个菌株处理的枳植株的株高、茎粗、叶片数和叶柄长均高于CK,是枳的优势菌株,能促进枳的生长。
关键词:丛枝菌根真菌;柑橘砧木;枳;菌根侵染率;促进生长
中图分类号:S666 文献标识码:A
Abstract: Bitter orange (Fructus aurantii) is commonly used as the rootstock for citrus. In this experiment, the arbuscular mycorrhizal fungi isolated from Guangxi soil were inoculated into the potting mix where bitter orange seedlings were grown, and the growth characteristics of mycorrhizal and non-mycorrhizal plants were compared. The data showed that all 14 arbuscular mycorrhizal fungi strains employed in the experiment could form symbiotic relationship with the root system of F. aurantii. The overall average infection rate was 36.16%, with Redeckera fulvum having the highest infection rate (92.83%). Glomus coronatum exhibited the lowest infection rate (0.23%). Redeckera fulvum, Glomus mosseae, and Glomus coronatum significantly increased plant height, stem diameter, leaf number, and petiole length of F. aurantii seedlings, when compared to the mock inoculation control group. The study demonstrates that the inoculation of the arbuscular mycorrhizal fungi could significantly promote the growth of bitter orange plants.
Keywords: arbuscular mycorrhizal fungi; citrus rootstock; Fructus aurantii; mycorrhizal infection rate; growth promotion
DOI: 10.3969/j.issn.1000-2561.2021.11.029
柑橘是全球最重要的经济作物之一,在水果贸易中排名第一位,我国是世界柑橘的重要原产区之一。柑橘是南方最主要的果树树种,对果农脱贫致富,农村经濟发展起着重要作用[1]。柑橘是典型的菌根依赖植物,其生长发育很大程度依赖于菌根的形成[2]。
丛枝菌根(arbuscular mycorrhizal,AM)真菌广泛分布于大自然的土壤中,地球上80%以上的陆生植物都能与AM真菌形成共生关系[3]。AM真菌不仅能促进植物对氮、磷、钾、钙、锌、铜等营养元素和水分的吸收[4-6],促进植物生长,还能提高植物对逆境(干旱、盐碱、重金属污染、病虫害等)的抗性[7-10]。虽然AM真菌对宿主植物具有综合的有益效应,但是不同AM真菌的功能不尽相同,对同一种宿主植物的效应也存在差异。……
