蔡燕 林婕 何金 杨福长 张冬梅 张连虎 王宗华

摘  要：小G蛋白MoRho3在稻瘟病菌生命活动中具有重要的生物学功能。前期研究表明，MoRho3的缺失对于稻瘟病菌分生孢子的形态建设、附着胞萌发和侵入能力以及对宿主的致病性等表型都存在严重缺陷。为了进一步解析MoRho3在稻瘟病菌中的网络调控途径，通过生物信息学的方法预测了MoRho3的互作蛋白。在众多假定互作蛋白中，蛋白激酶MoKin1与酿酒酵母中KIN1和KIN2是同源蛋白。在酵母细胞中，KIN1和KIN2可以抑制△rho3的表型缺陷。为了验证在稻瘟病菌中MoKin1与MoRho3之间是否存在着相似的调控途径，本研究通过分析MoKin1与同源蛋白的进化关系，序列结构，定位状态以及相关的互作蛋白，揭示了MoKin1的功能特点，为后续病原菌中MoKin1与MoRho3互作机制的研究，以及病原菌致病机制的解析提供了新的思路和方向。

关键词：稻瘟病菌;MoRho3;MoKin1;互作分析

中图分类号：S432.4+4      文献标识码：A

Abstract： Small GTPase MoRho3 plays an important biological role in the rice blast fungus， Magnaporthe oryzae. The preamble research elucidated that the deletion of MoRho3 in this fungus caused serve phenotypic defects， such as ab-normal morphology of conidia， reduction of germination and penetration of appressoria and reduced pathogenicity. In order to understand the mechanism of the regulatory pathways of MoRho3， we adopted the bioinformatics to search the putative interacting protein of MoRho3. We found the homologus protein of Saccharomyces cerevisiae KIN1 and KIN2 in the proteins， named MoKin1. Because the over-expression of KIN1 and KIN2 could rescue some phenotypic defects of △rho3 in S. cerevisiae， in order to verify whether there was a similar regulatory pathway between Mokin1 and MoRho3 in M. oryzae， the study revealed the functional characteristics of Mokin1 by analyzing the evolutionary relationship， sequence structure， localization status and related interaction proteins， which would provide a new basis for the further study of the interaction between Mokin1 and MoRho3 and the pathogenic mechanism of M. oryzae.

Keywords： Magnaporthe oryzae; MoRho3; MoKin1; interaction analysis

DOI： 10.3969/j.issn.1000-2561.2021.11.026

稻瘟病菌（Magnaporthe oryzae）引起的稻瘟病（rice blast disease）是水稻生产中最重要的真菌病害之一。每年引起水稻产量的损失数以亿计，造成严重的粮食危害[1]。因此，稻瘟病的防控是水稻生产上最重要的任务。水稻与稻瘟病菌的互作机制是植物与病原物互作的经典模型之一，推动了植物病原真菌致病机制和寄主植物抗病机制的研究[2]。目前，主要从水稻的抗病和稻瘟病菌的致病两个方面进行研究，为水稻抗病品种的培育以及新型杀菌剂的研发提供理论方面的指导。

稻瘟病菌的分生孢子随着风雨的传播，散落在健康的水稻叶表皮。……