庄俊红

摘要：目的：研究肿瘤患者外周血中淋巴细胞亚群的各项指标及CD39分子表达水平和肿瘤患者临床信息及预后的相关性。方法：选取宫颈癌患者共43例，收集肿瘤患者外周血并用流式細胞术检测和分析外周血中淋巴细胞亚群7项指标及CD39分子表达水平，同时收集肿瘤患者的临床信息，分析两者是否具有相关性。结果：研究发现宫颈癌患者外周血中淋巴细胞亚群7项指标，其中CD8阳性T细胞绝对数及CD4/CD8比值同患者的临床分期、淋巴结转移和远处转移相关;此外在宫颈癌中CD4阳性和CD8阳性T淋巴细胞表面CD39分子的表达水平同患者的临床分期、淋巴结转移和远处转移相关。结论：本研究发现宫颈癌早中期患者外周血中CD8阳性T细胞的数量较晚期患者明显增多，提示肿瘤患者外周血中CD8阳性T细胞的数量同患者的预后相关;此外早中期宫颈癌患者CD4阳性和CD8阳性T细胞表面CD39分子表达水平明显低于晚期患者，提示CD39分子可能同晚期患者CD8阳性T细胞数量减少相关。

关键词：淋巴细胞亚群，流式细胞术，细胞毒性T细胞

Abstract：Objective  The purpose of this study is to investigate the correlation between lymphocyte subsets， CD39 expression level in peripheral blood of tumor patients and their clinical information and prognosis. Methods Initially， We chose 134 patients who have one of the following cancers： lung cancer， cervical cancer， or breast cancer and collected their clinical information and peripheral blood. Furthermore， we detected 7 indicators of lymphocyte subsets and CD39 expression level in their peripheral blood by flow cytometry and analyzed the correlation between those indicator values of each individual and its corresponding clinical information. Results According to our study， correlation did exist between the absolute counts of CD8+ T cells， the ratio of CD4/CD8 and clinical stages， lymph node metastasis， distant metastasis;and also there is correlation between the CD39 expression level and clinical stages， lymph node metastasis， distant metastasis. Conclusion The number of CD8+ T cells in the peripheral blood of patients with cervical cancer in the early and middle stage is significantly higher than that inthe advanced stage， suggesting that the number of CD8+ T cells is associated with the prognosis of patients. In addition， the expression levels of CD39 molecules on the surface of CD4+ and CD8+ T cells in patients with early and middle stage cervical cancer were significantly lower than those in advanced patients， suggesting that CD39 molecules may be related to the decrease in the number of CD8+ T cells in advanced patients

Key Words：lymphocyte subsets， flow cytometry， cytotoxic T cell

【中图分类号】R711.74             【文献标识码】A             【文章编号】2107-2306（2021）12--03

人口的增长和老龄化，以及多种不良生活方式导致癌症患者数量越来越多，全球癌症负担持续增加。癌症的发生、发展与机体的免疫状态密切相关。机体抗肿瘤免疫中免疫细胞起主导作用，能够识别和清除肿瘤细胞。流式淋巴细胞亚群作为一种常规检查，是反映细胞免疫功能的重要指标，在肿瘤免疫监测具有重要意义，进一步为患者的临床治疗方案提供参考依据。……