解华云 艾辉建 洪日新 覃武 覃斯华 叶云峰 何毅 李桂芬 黄金艳 柳唐镜 李天艳 韦正光

摘 要：為了解薄皮甜瓜嫁接后的砧穗亲和力和砧穗愈合情况，以广西当地主栽品种广蜜1号为接穗，选择广西农业科学院园艺研究所选育的白瓜砧BX1、南瓜砧NX3、南瓜砧NX4等3个品系作为砧木，进行了嫁接后第2、4、6、8、10 d的砧穗愈合组织学观察。结果表明：白瓜砧BX1、南瓜砧NX3、南瓜砧NX4均表现出良好的嫁接亲和力，嫁接后砧穗愈合部位的组织学变化及愈合速度基本相同。嫁接后第2 d，砧木和接穗各自形成一层隔离层，嫁接体处于隔离层形成阶段;嫁接后第4 d，隔离层颜色变浅，隔离层的边缘形成愈伤组织，嫁接体处于愈伤组织形成阶段;嫁接后第6 d，隔离层染色颜色明显变淡，愈伤组织进一步分化，形成愈伤组织桥;嫁接后第8 d，砧木和接穗切口相接处形成许多间断的愈伤组织桥，隔离层外侧的大部分空隙被愈伤组织填充;嫁接后第10 d，砧木和接穗切口相接处的隔离层几乎完全消失。

关键词：薄皮甜瓜;嫁接;砧穗愈合;组织学

中图分类号：S 652 文献标志码：A 文章编号：0253-2301（2021）09-0059-05

DOI： 10.13651/j.cnki.fjnykj.2021.09.010

Histological Observation on the Healing Process of Grafted Rootstockand Scion of Thin Skinned Cucumis Melo

XIE Hua yunAI Hui jianHONG Ri xinQIN WuQIN Si hua ， YE Yun fengHE Yi

LI Gui fenHUANG Jin yanLIU Tang jingLI Tian yanWEI Zheng guang

（1. Institute of Horticulture， Guangxi Academy of Agricultural Sciences， Nanning， Guangxi 530007， China;

2. Guangxi Engineering Research Center of Muskmelon， Nanning， Guangxi 530007， China;

3. Nanning Technology Transformation Service Centre， Nanning， Guangxi 530007， China;

4. Institute of Plant Protection， Guangxi Academy of Agricultural Sciences， Nanning， Guangxi 530007， China）

Abstract： In order to understand the rootstock scion compatibility and healing of thin skinned Cucumis melo after grafting， Guangmi No.1， the main local cultivar in Guangxi， was selected as the scion， and the three cultivars， white melon rootstock BX1， pumpkin rootstock NX3 and pumpkin rootstock NX4， which were bred by Horticulture Institute of Guangxi Academy of Agricultural Science， were selected as the rootstocks. Then， the histological observation of the rootstock scion healing on the 2nd， 4th， 6th， 8th and 10th days after grafting was carried out. The resultsshowed that white melon rootstock BX1， pumpkin rootstock NX3 and pumpkin rootstock NX4 all showed good grafting compatibility， and the histological changes of the rootstock scion healing site and the healing speed after grafting were basically the same. On the 2nd day after grafting， the rootstock and scion formed an isolation layer respectively， and the graft was in the formation stage of isolation layer. On the 4th day after grafting， the color of the isolation layer became lighter， and the callus formed at the edge of the isolation layer， and the graft was in the formation stage of callus. On the 6th day after grafting， the color of the isolation layer became lighter obviously， and the callus differentiated further， forming callus bridges. On the 8th day after grafting， many discontinuous callus bridges were formed at the junction between the incisions of rootstock and scion， and most of the gap outside the isolation layer was filled with callus. On the 10th day after grafting， the isolation layer at the junction of rootstock scion incision almost disappeared.

Key words： Thin skinned Cucumis melo; Grafting; Rootstock scion healing; Histology

嫁接亲和力是指砧木与接穗嫁接以后能否成活，嫁接苗能否正常生长结果，它是选择适宜砧木的依据。国内一些学者对甜瓜嫁接后的田间抗病性及嫁接后对植株生长势、产量和品质的影响做了细致的研究。方胜等[1] 研究了不同砧木嫁接对甜瓜生长特性与品质的影响，筛选出嫁接亲和力高、抗病性强，且不影响甜瓜品质的砧用南瓜新土佐。……