石桃雄 黎瑞源 潘凡 黄娟 朱丽伟 汪燕 梁成刚

摘要：【目的】開展苦养籽粒总黄酮含量与SSR标记的关联分析，挖掘与黄酮含量相关的分子标记，为高黄酮含量苦荞品种的遗传改良提供依据。【方法】以193份苦荞种质为供试材料，基于62对SSR引物分析了供试种质的遗传多样性，对总黄酮含量和SSR标记进行了关联分析。【结果】193个种质籽粒总黄酮含量的变幅为1.04%- 2.99%，变异系数为27.93%。62对引物在193个种质中共扩增出267个等位基因，每个SSR位点平均检测到245个有效等位基因，基因多样性为0 503，Shannon信息指数为0 942，观测杂合度为0.513，引物多态信息量为0.74。群体结构分析将193份种质划分为3个亚群。基于广义线性模型（ GIM）共检测到5个与籽粒总黄酮含量显著关联的SSR标记，表型贡献率为6.3%- 12.9%，其中标记TatG0124（ 12.9%）和S6853（ 8.5%）的表型贡献率较高。【结论】供试苦荞种质遗传多样性丰富，可用于苦荞重要农艺和品质性状的关联分析。TatG0124和SSR6853可能是控制籽粒黄酮含量的重要位点，对改良苦荞籽粒黄酮含量具有重要意义。关键词：苦荞;SSR标记;黄酮含量;遗传多样性;群体结构;关联分析

中图分类号：S 517

文献标志码：A

文章编号：1008-0384（ 2021） 08088408

Correlation Between Flavonoids Content and

SSR Markers of Tartary Buckwheat

SHI Taoxiong. LI Ruiyuan. PAN Fan， HUANG Juan. ZHU Liwei. WANG Yan. LIANG Chenggang*

（ Buckwheat Industry Technology Research Center. Guizhou Normal Universily， Guiyang， Guizhou 55000I . China ）

Abstract： 【Objective】 Correlation between the flavonoids content and associated markers was analyzed for geneticimprovement studies on Tartary buckwheat. 【Method】Sixty-two SSR primer pairs were used to assess the genetic diversityof 193 Tartary buckwheat germplasms. The SSR markers closely related to the total flavonoids content were selected byassociation analysis. 【Result】The total flavonoids content of 193 Tartary buckwheat germplasms varied from l.04% to2.gg% with a coefficient variation of 27.93%. Sixty-two pairs of SSR primers amplified 267 polymorphic sites with an averagenumber of effective alleles of 2.45. The mean values of gene diversity， Shannon index. observed heterozygosity andpolymorphism information content of SSR markers were 0.503. 0.942. 0.5 13 and 0.74. respectively. The population structureanalysis divided the 193 germplasms int0 3 subgroups. Five SSR markers were determined to be significantly associated withflavonoids content by the general linear model （GLM）. The phenotypic variations ranged from 6.3% t0 12.9% with TatG0124at 12.9% and S6853 at 8.5% being the higher markers. 【Conclusion】The germplasms used in this study were diverse ingenetic variation and feasible for related genome-wide mapping on important traits of Tartary buckwheat. The loci. TatG0124and S6853， appeared to closely associate with the flavonoids content of Tartary buckwheat and were considered to bepotentially useful for breeding new varieties with high content of the functional components.

Key words： Tartary buckwheat： SSR marker; flavonoids content; genetic diversity; population structure; correlation analysis

0 引言

【研究意义】苦荞（Fagopyrum tataricum），又名鞑靼荞麦（ Tartary buckwheat），属蓼科（Polygonaceae）荞麦属（Fagopyrum Mill）雙子叶植物，是荞麦的两大栽培品种之一[1]，广泛种植于云、贵、川、藏等省区的高原和高寒地带[2]。苦荞是我国重要的杂粮作物之一，其籽粒含有丰富的多酚类化合物、抗性淀粉、可溶性纤维、蛋白质、氨基酸、维生素及微量元素等，可有效地预防和改善肥胖、胆结石和心血管等疾病[3-4]，在食品、保健品、医药等领域具有广泛的应用前景。……