张燕丽 岳秋娟 陈香君 张敏

摘要：目的 初步探讨藏药水母雪莲SMM（Saussurea medusa Maxim）的抗肿瘤免疫功能，为进一步开发水母雪莲药用价值提供科学依据。方法 建造lewis荷瘤小鼠模型后，随机分为生理盐水组（normal saline，NS）、SMM黄酮类组和环磷酰胺（Cyclophosphamide，CTX）组，体外治疗14天。以lewis荷瘤小鼠肿瘤组织HE形态染色以及血清中IL-2和IFN-γ为检测指标。结果 lewis荷瘤小鼠经SMM黄酮类提取物治疗后，肿瘤浸润组织部位的肿瘤细胞出现局灶性或者片状坏死并伴有新生血管形成;还提高了荷瘤小鼠血清中IL2和IFN-γ的含量。结论 水母雪莲黄酮类提取物有一定抗肺癌免疫治疗的效应。

关键字：水母雪莲;抗肿瘤效应

[Abstract] Objective To preliminarily explore the anti-tumor immune function of Saussurea medusa Maxim （Saussurea medusa Maxim）， and provide scientific basis for further development of the medicinal value of Saussurea medusa Maxim. Methods After the lewis tumor-bearing mouse model was constructed， they were randomly divided into normal saline ， SMM flavonoids and cyclophosphamide groups. They were treated in vitro for 14 days.The HE morphological staining of tumor tissue and serum IL-2 and IFN-γ in Lewis tumor-bearing mice were used as the detection indicators. Results After lewis tumor-bearing mice were treated with SMM flavonoids extract， focal or patchy necrosis of tumor cells and neovascularization were found in tumor infiltrating tissues; it also increased the levels of IL2 and IFN-γ in serum of tumor-bearing mice. Conclusion The flavonoid extract of Saussurea medusa has a certain anti-lung cancer immunotherapy effect.

肿瘤是严重威胁人类健康的一种疾病。其中肺癌的发病率逐年增高，也是致死率最高的癌症之一。其主要治疗方法包括手术、放疗和化疗等，但是该治疗方案会导致患者免疫功能紊乱，预后差[1]。近几年随着人们对肿瘤治疗的不断研究和深入，发现在恶性肿瘤的综合治疗中，中医药可以通过调节机体的免疫机制来发挥抗肿瘤作用且毒副作用小[2]。因此本文采用lewis荷瘤小鼠为研究对象，初步探讨藏药水母雪莲黄酮类的抗肿瘤作用，以期为肿瘤临床治疗及靶向药物开发提供新的思路。

1 材料与方法

1.1 实验动物及主要材料

SPF级野生型C57BL/6小鼠（6～8周龄、20～25 g）购于达科为生物公司，饲养于西藏民族大学实验动物科学部。Lewis肺癌细胞株3LL由上海复旦大学赠予。水母雪莲选择西藏山南野生品种。CTX购自江苏恒瑞制药公司。多聚甲醛、二甲苯购于武汉科瑞生物技术有限公司。……