翟孟凡 贾小强 王泽鹏 张娇

摘要 目的：通过运用网络药理学方法来探究“黄芪-白术”治疗功能性便秘（FC）的可能机制。方法：运用网络数据库对黄芪-白术药对有效成分及FC靶点进行检索筛选。运用作图软件构建“黄芪-白术”药对的“成分-靶点”网络图。利用STRING数据库构建黄芪-白术药对治疗便秘的蛋白质-蛋白质相互作用（PPI）网络图，拓扑分析出核心靶点。最后，通过Metascape数据库对药物-疾病公共靶点进行生物过程及通路富集分析。结果：在黄芪-白术药对中共筛选出27个与FC相关的有效成分和497个潜在作用靶点;拓扑分析得到16个核心靶点;富集分析得到20条富集结果，其中基因本体论（GO）生物过程的细胞对激素刺激的反应上靶点富集数目最多。结论：黄芪-白术药对治疗FC具有多成分、多靶点、多途径的特点，其机制可能主要通过对细胞对激素刺激反应的调控来实现，也提示该通路是未来研究黄芪-白术药对治疗FC机制的重要方向。

关键词 网络药理学;黄芪-白术;药对;功能性便秘;机制研究

Abstract Objective：To explore the possible mechanism of Astragalus membranaceus-Atractylodes macrocephala in the treatment of functional constipation（FC）by network pharmacology.Methods：The effective components and FC targets of Astragalus membranaceus and Atractylodes macrocephala drug pair were searched and screened by using network database.By using STRING database to construct protein interaction network（PPI）of Astragalus membranaceus-Atractylodes macrocephala drug pair for constipation treatment，the core targets were topologically analyzed.Finally，bioprocess and pathway enrichment analysis of drug-disease public targets was made through Metascape database.Results：A total of 27 FC-related active ingredients and 497 potential targets were screened out from the Astragalus-Atractylodes macrocephala drug pair.By topological analysis，16 core targets were obtained.A total of 20 enrichment results were obtained by enrichment analysis.Among them，GO（gene ontology，GO）biological process cells had the largest number of targets enriched in cellular response to hormone stimulus（cellular response to hormone stimulus）.Conclusion：Astragalus-Atractylodes macrocephala drug pair is multi-component，multi-target and multi-channel in the treatment of FC，and its mechanism may be mainly realized through the regulation of cell response to hormone stimulation.It also suggests that this pathway is an important direction for future research on the mechanism of Astragalus-Atractylodes Macrocephala drug in the treatment of FC.

Keywords Network pharmacology; Astragalus-Atractylodes Macrocephala; Drug pair; Functional Constipation; Mechanism research

中圖分类号：R242;R574文献标识码：Adoi：10.3969/j.issn.1673-7202.2021.10.003

功能性便秘（Functional Constipation，FC）是便秘的常见类型，其临床表现主要为排便费力，排便次数减少，排便不尽感等，其发病因素与排除明确的药物性因素和肠道器质性因素等因素有关[1]。FC是一种临床常见病，多发病，在女性和中老年人中发病率更高[2]。黄芪-白术药对治疗功能性便秘效果确切，并有广泛运用，但其具体作用成分和作用靶点尚未见到相关报道。最近几年，网络药理学为研究中药药效的具体作用机制提供了思路，因其具有以“疾病、基因、药物、靶点”为基础的特色[3]。……