范志毅 罗聪 余海霞 曾学梅 王金英 谢小杰 何新华

摘  要：FY是拟南芥自主途径中调控成花的基因，FY通过与RNA结合蛋白FLOWERING CONTROL LOCUS A （FCA）相互作用下调成花抑制基因FLOWERING LOUS C （FLC）的表达从而促进开花。目前关于FY基因如何调控芒果成花的机制尚无研究报道。通过挖掘芒果转录组数据克隆获得1个FY基因，命名为MiFY。生物信息学分析显示，MiFY基因的ORF全长为2178 bp，编码725个氨基酸，蛋白质分子量为799.59 kDa，理论等电点为8.72，氨基酸序列中含有WD40和Cytadhesin_P30两个保守结构域。基因表达模式分析显示，MiFY基因在2个芒果品种的开花期均具有较高的表达水平，而在营养生长期表达水平较低。因此推测MiFY基因可能在调节芒果成花中起着重要作用。

关键词：芒果;开花基因;FY;克隆;表达模式

Abstract： FY is a flowering gene in the autonomous pathway of Arabidopsis thaliana. The expression of the flower suppressor gene FLC is down regulated by interaction with the RNA binding protein FLOWERING CONTROL LOCUS （FCA）. The mechanism of how FY gene regulating the flower formation in mango has not been studied. In the study， a FY homologous gene was obtained from the transcriptome data of mango， named MiFY. Sequences analysis showed that the open reading frame of the gene had 2178 bp which encoding 725 amino acids. The molecular weight was 799.59 kDa and isoelectric point was 8.72， respectively. Conserved domain analysis showed that FY protein contained two conserved domains， WD40 and Cytadhesin_P30. Expression analysis showed that MiFY expressed in all tested tissues but with expression level differences. MiFY gene was higher expressed during the flowering development period and lower expressed during the vegetative growth period. The results indicated that MiFY gene may play an important role in regulating mango flowering.

Keywords： mango; flowering gene; FY; cloning; expression pattern

成花轉变是高等植物生长发育重要的生理过程之一。通过对模式植物拟南芥成花分子机制的研究发现，参与植物成花调控的途径包括光周期途径、春化途径、赤霉素途径、年龄依赖途径、温敏途径和自主途径[1]。在没有外源开花信号诱导的条件下，植物的营养生长达到一定阶段后也会开花的途径叫做自主途径[2]。其中，拟南芥的自主途径通过下调成花抑制基因FLOWERING LOUS C（FLC）的表达而促进开花[3]。目前自主途径至少包括7个基因：FY、FLOWERING CONTROL LOCUS A （FCA）、FLOWERING LOCUS D （FLD）、FLOWERING LOCUS K （FLK）、LUMINIDEPENDENS （LD）、FPA、FVE，任何一个基因的突变都会增加FLC的表达，导致开花延迟[4-5]。其中，FY基因作为一个高度保守的开花时间调控基因，是一个具有特殊C末端的真核蛋白聚腺苷酸化特异性因子（leavage and polyadenylation specificity factor，CPSF）复合物的亚单位，FY基因的C末端有2个植物独有的Pro-Pro-Leu-Pro-Pro（PPLPP）结构域，可……