张敏 刘惠娟 王永福 石岩

摘要 摘要[目的]建立一测多评法同时测定淫羊藿中6种黄酮类成分的方法，并对甘肃地区的21批药材进行定量分析。[方法]Agilent Zorbax色谱柱（4.6 mm×250 mm，5 μm），流动相为水（A）、乙腈（B），梯度洗脱，流速0.8 mL/min，检测波长270 nm，进样量10 μL。分别考察不同条件对相对校正因子的影响。评价一测多评法和标准曲线法在含量测定结果上的差异。[结果]建立了以淫羊藿苷为内标参照物，同时测定6种黄酮类成分的一测多评法。不同条件下相对校正因子的RSD小于2.63%。多点矫正-一测多评法、斜率矫正-一测多评法和标准曲线法测定21批甘肃地区淫羊藿样品含量，其RSD小于3%，无明显差异。[结论]一测多评法结果准确、操作简单易行，可用于淫羊藿中黄酮类成分的含量测定。

关键词 一测多评;淫羊藿;黄酮类成分;含量测定;甘肃地区

中图分类号 R-284.1文献标识码 A文章编号 0517-6611（2021）11-0182-04

doi：10.3969/j.issn.0517-6611.2021.11.049

开放科学（资源服务）标识码（OSID）：

Determination of Flavonoids of Epimedium in Gansu by Quantitative Analysis of Multi-components with a Single Marker（QAMS）

ZHANG Min1， LIU Hui-juan1， WANG Yong-fu2 et al

（1.Gansu Medical College， Pingliang，Gansu 744000;2.Gansu Digital Herbal Testing Center Co.， Ltd.， Dingxi，Gansu 743000）

Abstract [Objective]To establish a method for simultaneous determination of 6 flavonoids in Epimedium by QAMS， and to determine the content of 21 batches of medicinal materials in Gansu Province. [Method]Agilent Zorbax column （4.6 mm×250 mm， 5 μm）， mobile phase were water （A） and acetonitrile （B）， gradient elution， flow rate was 0.8 mL/min， the detection wavelength was set at 270 nm， injection volume 10 μL.The effects of different conditions on relative correction factors were investigated.The difference between the standard curve method and QAMS was evaluated in content determination. [Result] A method was established to determine 6 flavonoids by using icariin as internal reference based on QAMS.The RSD under different conditions was less than 2.63%. The content of 21 batches of Epimedium in Gansu Province was determined by QAMS and standard curve method， the RSD of two sets was less than 3% and there was no significant difference. [Conclusion] QAMS is accurate， easy to operate， and can be used to determine the content of flavonoids in Epimedium.

Key words QAMS;Epimedium;Flavonoids;Content determination;Gansu area

淫羊藿為小檗科植物淫羊藿Epimedium brevicornu Maxim.、箭叶淫羊藿Epimedium sagittatum（Sieb.et Zucc.）Maxim.、柔毛淫羊藿Epimedium pubescens Maxim.或朝鲜淫羊藿Epimedium koreanum Nakai的干燥叶[1]，其基源复杂、分布广泛、质量差别大[2]。而甘肃地区的淫羊藿质量一直为市场所认可[3]。

淫羊藿的主要药效成分为黄酮类，国内外大量研究都集中在此类成分研究上[4-5]。2015版《中国药典》以淫羊藿苷≥0.5%作为含量合格的指标，造成市面上多数药材质量不合格[6]。现代研究表明，淫羊藿中具有生理活性的成分不局限在淫羊藿苷。朝藿定C抗骨质疏松效果明显，宝霍苷I对鼻咽癌、口腔上皮癌有作用[7-8]。因此，全面考察淫羊藿中黄酮类成分，才能科学地反映药材质量。……