摩西球囊霉(Glomus mosseae)与“津研4号”黄瓜的共生效应研究
2021-07-16何佳怡樊娅萍李东林王倡宪
何佳怡 樊娅萍 李东林 王倡宪



摘要 采用盆钵培养的方法,以蛭石为基质培育“津研4号”黄瓜幼苗,育苗时接种摩西球囊霉(Glomus mosseae),并浇灌Hoagland营养液。结果表明,随着营养液浓度的升高,该AM真菌与“津研4号”可以形成共生体,但二者的亲和力较低,28 d苗龄时菌根侵染率仅为26.5%。接种G.mosseae的黄瓜幼苗根系全磷含量显著高于对照,幼苗质量与对照间无显著差异。
关键词 津研4号;摩西球囊霉;亲和力;幼苗质量
中图分类号 S-642.2 文献标识码 A
文章编号 0517-6611(2021)12-0176-04
doi:10.3969/j.issn.0517-6611.2021.12.045
开放科学(资源服务)标识码(OSID):
Study on Symbiotic Effect of Glomus mosseae and Jinyan No.4 Cucumber (Cucumis sativus L.)
HE Jia yi,FAN Ya ping,LI Dong lin et al (College of Modern Agriculture and Ecological Environment,Heilongjiang University,Key Laboratory of Agricultural Resources Utilization and Environmental Security,Harbin,Heilongjiang 150080)
Abstract A pot experiment was carried out,vermiculite was used as the nursery substrate,and Jinyan No.4 cucumber seedlings inoculated with G.mosseae were irrigated by Hoagland nutrients solution at nursery stage.The results showed that G.mosseae and Jinyan No.4 formed a symbiosis with the increase of nutrient solution concentration,but the affinity between the partners was low,and the mycorrhizal infection rate was only 26.5% at 28 d seedling age.The phosphorus concentration in roots of seedlings inoculated with G.mosseae was higher than that of control,and there was no significant difference in seedlingss qualities between the two treatments.
Key words Jinyan No.4;G.mosseae;Affinity;Seedlingss quality
叢枝菌根真菌(arbuscular mycorrhizal fungi,简称为AMF)隶属于球囊霉门,是一类活体营养真菌,该类真菌可以侵染80%~90%的陆生维管植物,进而形成具有双膜结构的菌根共生体[1-3]。共生体系建立后,AM真菌代谢所需碳水化合物与脂质由寄主供给,与此同时,AM真菌协助寄主获取生长所需矿质养分[2,4]。
黄瓜是我国设施栽培的主要蔬菜种类之一,栽培面积逐年增加,栽培各环节中培育壮苗是获得高产的关键。研究证实,AM真菌与黄瓜可形成互惠共生体,育苗时接种适宜的AM真菌不仅能促进黄瓜生长而且对寄主抗逆能力的改善有积极作用[5-12]。笔者选取目前抗霜霉病与白粉病能力较强的露地主栽品种“津研4号”为寄主,于黄瓜育苗时接种农田中广泛分布的AM真菌Glomus mosseas,以明确该黄瓜品种与AM真菌的共生效应,以期为黄瓜育苗时合理筛选适宜的共生体组合提供理论依据。
1 材料与方法
1.1 试验材料 黄瓜品种:
试验所用黄瓜品种为“津研4号”(购自天津科润黄瓜研究所),先将种子浸泡于10% H2O2中表面消毒5 min,然后用灭菌的蒸馏水冲洗干净,温汤浸种(50~55 ℃持续搅拌15 min),最后放入铺有湿润滤纸的培养皿中黑暗催芽。……
