刘丹 尚春明 杨进 张仙保 潘子旺 刘燕 郑于莉

摘要 以根结线虫病样、番茄品种及组合为试材，采集包头市根结线虫暴发地一个根结线虫病样，进行形态学和分子生物学鉴定;利用PCR技术对24个番茄品种及组合进行Mi-1基因扩增筛选。结果表明，感染内蒙古包头市番茄的根结线虫为南方根结线虫;筛选出3个纯合抗根结线虫番茄组合。抗感品种及组合均产生750 bp的特异片段，纯合抗病基因的番茄组合存在TaqⅠ酶切位点，酶切后产生了570和160 bp特异性片段，为防治当地番茄根结线虫及培育抗病品种积累材料。

关键词 番茄;根结线虫;分子生物学

中图分类号 S-433.1  文献标识码 A  文章编号 0517-6611（2021）12-0148-04

doi：10.3969/j.issn.0517-6611.2021.12.037

开放科学（资源服务）标识码（OSID）：

Pathogen Identification and Resistant Material Screening of Tomato Root knot Nematode in Protected Field

LIU Dan1， SHANG Chun ming1， YANG  Jin2 et al

（1.Baotou Institute of Agricultural Science， Baotou， Inner Mongolia 014013; 2. Inner Mongolia Agricultural University， Baotou， Inner Mongolia  014013）

Abstract Taking the root knot nematode samples and tomato varieties and combinations as test materials， a root knot nematode disease sample from the root knot nematode outbreak area in Baotou City was collected for morphological and molecular biological identification. The Mi 1 gene amplification and screening of 24 tomato varieties and combinations were carried out by PCR technology. The results showed that the root knot nematode infected tomato in Baotou City of Inner Mongolia was the southern root knot line， three homozygous tomato combinations with resistance to root knot nematode were screened out. The resistant varieties and combinations all produced 750 bp specific fragments. The Taq I restriction site existed in the homozygous resistant tomato combinations， and 570 and 160 bp specific fragments were produced after enzyme digestion， which were used to accumulate materials for the control of local tomato root knot nematode and the cultivation of disease resistant varieties

Key words Tomato;Root knot nematode;Molecular biology

根結线虫是一类在农作物上危害极大的植物寄生性线虫。据不完全统计，世界各地农作物因根结线虫危害，平均每年可使农业减产24.5%，造成经济损失在1 000亿美元以上[1]。目前，报道近百种根结线虫有效种，能够寄生的植物达3 000多种，其中最常见造成危害最严重的线虫有南方根结线虫、北方根结线虫、爪哇根结线虫、花生根结线虫[2]。

近年来，随着种植结构的调整，保护地蔬菜复种指数越来越高，致使根结线虫病危害日趋严重，造成蔬菜大幅减产[3]。保护地番茄栽培面积相对较大，抗根结线虫病的番茄品种相对缺乏，已成为生产上急需解决的问题[4]。内蒙古自治区随着蔬菜供应量不断增加，根结线虫也迅速发展和蔓延[5]。根结线虫已成为一类严重危害番茄且具有广泛寄主的植物寄生线虫。……