李博文　李玲敏　夏萌　潘思媛　陈佳灜　娄玉霞　明凤

摘  要： 為探究外源吲哚丁酸（IBA）对月季鲜切花的保鲜效应，采用蔗糖+水杨酸（SA）为基础保鲜液，分别添加不同浓度梯度的IBA，对月季‘黑魔术鲜切花进行瓶插保鲜处理，观察分析鲜切花的各项生理指标.结果表明：保鲜处理12 d后，不同浓度IBA对鲜切花的生长和品质产生了不同的影响.IBA质量浓度为250 mg?L^-1的保鲜液处理组，鲜切花完全萎蔫，丧失观赏价值.IBA质量浓度为500 mg?L^-1的保鲜液处理组中，鲜切花能维持较好的花瓣形态，花朵于第6 d进入盛花期，绽放冠幅达8 cm，且最终花瓣萎蔫率仅为24.3%.同时，该处理组较其他组生理状态更佳，水分平衡值和鲜重维持在较好的水平，超氧化物歧化酶（SOD）和过氧化物酶（POD）的活性提高，花瓣中蔗糖含量显著高于其他处理组.进一步分析影响花瓣脱落相关基因的表达，其中RcSUC2，RcARF7因外源施加了蔗糖和生长素，导致体内基因表达相对于对照组显著降低，而乙烯与生长素的拮抗作用也使得RcETR1的表达显著降低.基于上述研究结果，筛选获得对月季‘黑魔术鲜切花保鲜效果最佳的保鲜液配方.研究结果为月季切花保鲜应用提供了理论依据和技术参考.

关键词： 月季鲜切花; 吲哚丁酸（IBA）; 保鲜液; 保鲜效应

中图分类号： Q 945    文献标志码： A    文章编号： 1000-5137（2021）02-0243-10

Effect of IBA on its fresh-keeping of the cut roseRose hybrida Hort

LI Bowen^1，2?， LI Lingmin^1，2?， XIA Meng^1，2?， PAN Siyuan^1，2，CHEN Jiaying^1，2*， LOU Yuxia^1，2*， MING Feng^1，2*

（1.College of Life Sciences， Shanghai Normal University， Shanghai 200234， China;2.Development Center of Plant Germplasm Resources， Shanghai Klormal University， Shanghai 200234， China）

Abstract： In order to explore the fresh-keeping effect of exogenous indole butyric acid （IBA） on fresh cut rose，sucrose+salicylic acid （SA） was used as the basic fresh-keeping solution，and with different concentration of IBA，the fresh cut rose，Rose hybrida Hort were vase-holding treated.And the physiological indicators of the fresh cut rose were observed and analyzed.The results showed that after 12 days of fresh-keeping treatment，different concentrations of IBA had different effects on the growth and quality of cut flowers.In the fresh-keeping liquid treatment group with 250 mg?L^-1IBA content，the fresh cut flowers completely wilted and lost their ornamental value.In the fresh-keeping solution treatment group with 500 mg·L^-1IBA content，fresh cut flowers can maintain good petal form.The flowers entered the full bloom on the 6th day，with a blooming crown of 8 cm diameter，and the final petal wilting rate was only 24.3%.At the same time，this treatment group has better physiological status than other groups，maintaining a good water balance and fresh weight，increasing the activity of superoxide dismutase （SOD） and peroxidase （POD），and sucrose content in petals significantly higher than other treatment groups.Further analysis of the expression of genes related to petal shedding was carried out.Among them，RcSUC2andRcARF7were exogenously applied with sucrose and auxin，resulting in a significant decrease in gene expressionin vivocompared to the control group，and the antagonism of ethylene and auxin also significantly decreased the expression ofRcETR1.Based on the above research results，the formula of the preservation solution has the best preservation effect on cut roseRosa hybridaHort.The results of this study provide theoretical basis and technological support for the application of cut rose fresh-keeping.

Key words： fresh cut rose; indole butyric acid（IBA）; preservative solution; fresh-keeping effect

0  引言

月季（Rosa chinensis Jacq.）為蔷薇科、蔷薇属植物，是常绿、半常绿低矮灌木，条件适宜时四季开花.作为世界四大切花之一，月季色彩艳丽、香气浓郁、观赏性强，深受大众青睐.随着人们生活水……