杨若琪 王静 张颖颖

摘要 [目的]评估苦参碱对变形链球菌致龋性（如生长、产酸、生物被膜形成和细菌代谢活性）的影响。[方法]通过结晶紫染色测定苦参碱对生物被膜形成的抑制作用与已成熟生物被膜的根除作用，通过pH计测定苦参碱对变形链球菌产酸的影响，通过MTT法测定了苦参碱对变形链球菌浮游细胞与生物被膜细胞代谢活性的影响。[结果]苦参碱能够显著抑制变形链球菌的生长与生物被膜的形成，对已成熟的生物被膜也有根除作用。苦参碱处理后变形链球菌有机酸的产生明显减少，同时变形链球菌浮游细胞和生物被膜细胞的代谢活性也受到了抑制。[结论]苦参碱可以抑制致龋性生物被膜，是预防龋齿的候选药物之一。

关键词 苦参碱;变形链球菌;生物被膜;抑制作用

中图分类号 R285 文献标识码 A

文章编号 0517-6611（2021）09-0168-04

doi：10.3969/j.issn.0517-6611.2021.09.045

Abstract [Objective]To evaluate the effect of matrine on the cariogenicity of Streptococcus mutans （such as growth，acid production，biofilm formation and bacterial metabolic activity）.[Method]The inhibitory effect of matrine on the formation of biofilms and the eradication of mature biofilms were measured by crystal violet staining.The effect of matrine on the acid production of S.mutans was measured by a pH meter.The effect of matrine on the metabolic activities of S.mutans planktonic cells and biofilm cells was determined by MTT method.[Result]Matrine could significantly inhibit the growth of S.mutans and the formation of biofilms，and it could also eradicate mature biofilms.After treatment with matrine，the production of organic acids by S.mutans was significantly reduced，and the metabolic activities of its planktonic cells and biofilm cells were also inhibited.[Conclusion]Matrine can inhibit cariogenic biofilms and is one of the candidate drugs for preventing dental caries.

Key words Matrine;Streptococcus mutans;Biofilm;Inhibitory effect

盡管如今的牙科技术发展迅速，但是许多人依然无法摆脱牙齿疾病的痛苦。有数据显示，牙齿疾病每年给全球造成的经济损失高达2 980亿美元，占全球医疗总支出的4.6%[1]。龋齿是目前最常见的牙齿疾病之一。变形链球菌（Streptococcus mutans）是一种革兰氏阳性细菌，被认为是主要的致龋病原体[2]。变形链球菌能够代谢食物中的糖类并产生有机酸，从而腐蚀牙釉质的表面，导致龋齿的形成[3]。同时，变形链球菌在牙齿表面形成的生物被膜（也称为牙菌斑）也是引起龋齿的重要原因之一[4]。

生物被膜是包裹在自身产生的胞外聚合物基质中的微生物聚集体。……