张瑞 林明玥 王晨鑫 李雨帆 尹怡静 赵兴刚 邹琴

摘要 [目的]研究含多种中药成分的抗菌喷雾的体外抗菌效果并对其抗菌机理进行分析。[方法]选用大肠杆菌（E.coli）和金黄色葡萄球菌（S.aureus）2种菌，用肉汤稀释法和荧光染色法测定抗菌喷雾对其抑菌活性、抑菌率、最小抑菌浓度（MIC）和最低杀菌浓度（MBC），用滤纸片法测定其抑菌环，用扫描电子显微镜（SEM）和透射电子显微镜（TEM）观察抗菌喷雾对细菌表面结构和超微结构的影响。[结果] 中药抗菌喷雾在极短的时间内对E.coli和S.aureus的抑菌率就能达到100%，滤纸法显示抑菌环明显;MIC和MBC结果说明了中药抗菌喷雾在稀释5倍之后仍对2种细菌具有抑制作用;喷雾作用后的细菌大量死亡，表面及内部结构均发生明显变化。[结论]多种中药成分的抗菌喷雾对E.coli和S.aureus具有显著的联合抑菌效果。

关键词 中药抗菌喷雾;植物抗菌;抗菌活性;荧光染色;超微结构;抗菌机理

中图分类号 R285文献标识码 A文章编号 0517-6611（2021）10-0167-07

doi：10.3969/j.issn.0517-6611.2021.10.045

开放科学（资源服务）标识码（OSID）：

Antibacterial Performance Test of Traditional Chinese Medicine Antibacterial Spray in Vitro

ZHANG Rui，LIN Ming-yue，WANG Chen-xin et al

（Research Center for Nano-Biomaterials，Analytical & Testing Center， Sichuan University，Chengdu，Sichuan 610065）

Abstract [Objective] To study the in vitro antibacterial effect of an antibacterial spray which contains several kinds of traditional Chinese medicine and analyze its antibacterial mechanism.[Method]Escherichia coli （E.coli） and Staphylococcus aureus （S.aureus） were selected to determine the antibacterial activity， antibacterial rate， minimum inhibitory concentration （MIC） and minimum bactericidal concentration （MBC） of antibacterial spray by broth microdilution method and fluorescence staining method. The inhibition zone was determined by filter paper method. The effects of antibacterial spray on the surface structure and ultrastructure of bacteria were observed by scanning electron microscope （SEM） and transmission electron microscope （TEM）. [Result] The antibacterial rate of traditional Chinese medicine antibacterial spray against E.coli and S.aureus could reach 100% in a very short time. Filter paper method demonstrated obvious inhibition zone. The results of MIC and MBC indicated that the traditional Chinese medicine antibacterial spray against E.coli and S.aureus still had two kinds of bacteria inhibition effect after 5 times dilution. After spraying， a large number of bacteria died， and the surface and internal structure changed evidently. [Conclusion]The antibacterial spray of various kinds of Chinese medicine has remarkable bacteriostatic effect on E.coli and S.aureus.

Key words Traditional Chinese medicine antibacterial spray;Plant antibacterial;Antibacterial activity;Fluorescent staining;Ultrastructure;Antibacterial mechanism

抗生素的發现与应用使多种由致病菌引起的细菌感染性疾病有了很好的控制和治疗效果，大大地降低了人类细菌感染引起的发病率和死亡率。但抗菌药物在发挥强大抗菌功效的同时，致病菌也不断对现有药物产生适应性，即耐药性[1]。天然中草药因具有毒性低、抗菌范围广、不易产生耐药性、价格低廉等优点而成为研究的热点。此次试验所使用的中药抗菌喷雾，其主要成分为乙醇、苯扎氯铵和多种中草药（薄荷浸提液、黄芩提取物、苦参提取物）。……